Environmental genome shotgun sequencing of the Sargasso Sea
Environmental genome shotgun sequencing of the Sargasso Sea
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DOI：
10.1126/science.1093857
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发表时间：
2004-04-02
期刊：
影响因子：
56.9
通讯作者：
Smith, HO
中科院分区：
文献类型：
--
作者：
Venter, JC;Remington, K;Smith, HO
我们已经将“全基因组shot弹枪测序”应用于与从百慕大附近Sargasso Sea收集的海水样品的切向流量和撞击过滤器收集的微生物种群。总共生成，注释和分析了总共10.45亿个非冗余序列，以阐明这些环境样品中生物体的基因含量，多样性和相对丰度。根据序列相关性，这些数据估计至少来自1800种基因组物种，包括148种以前未知的细菌系统型。我们已经确定了这些样品中代表的120万以前未知的基因，其中包括782多个新型杜鹃素样光感受器。存在和化学计量的物种变化表明大量海洋微生物多样性。
We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity.