Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility
Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility
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DOI：
10.1111/imb.12604
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发表时间：
2019-07-03
影响因子：
2.6
通讯作者：
Sinkins, S. P.
中科院分区：
文献类型：
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作者：
Ant, T. H.;Herd, C.;Sinkins, S. P.
致倦库蚊（Culex quinquefasciatus）是人类和动物的许多病毒和原生动物病原体的重要蚊媒，并且天然携带内共生体Wolbachia pipientis，菌株wPip。沃尔巴克氏体用于两种不同的媒介控制策略：第一，群体抑制所造成的大量释放的transinfected男性和野生女性之间的交配不相容性;第二，传播的病原体传播阻断菌株通过人口。利用胚胎显微注射技术，在C.使用白纹伊蚊的天然菌株对致倦伊蚊进行的感染：wAlbB单一感染和wPip加wAlbA双重感染。wAlbB感染显示与野生型C完全双向胞质不相容性（CI）。quinquefasciatus在相互杂交。wPipwAlbA重叠感染显示完全单向CI，因此具有群体入侵潜力。尽管wAlbB菌株显示出与天然wPip类似的相对较低的总体密度，但与天然wPip相比，wPipwAlbA超感染在唾液腺中达到了超过400倍的高密度，这表明它可能是病原体传播阻断的候选者。
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population suppression caused by mating incompatibilities between mass-released transinfected males and wild females; and secondly, the spread of pathogen transmission-blocking strains through populations. Using embryonic microinjection, two novel Wolbachia transinfections were generated in C. quinquefasciatus using strains native to the mosquito Aedes albopictus: a wAlbB single infection, and a wPip plus wAlbA superinfection. The wAlbB infection showed full bidirectional cytoplasmic incompatibility (CI) with wild-type C. quinquefasciatus in reciprocal crosses. The wPipwAlbA superinfection showed complete unidirectional CI, and therefore population invasion potential. Whereas the wAlbB strain showed comparatively low overall densities, similar to the native wPip, the wPipwAlbA superinfection reached over 400-fold higher densities in the salivary glands compared to the native wPip, suggesting it may be a candidate for pathogen transmission blocking.