An improved sensitive assay for the detection of PSP toxins with neuroblastoma cell-based impedance biosensor.
An improved sensitive assay for the detection of PSP toxins with neuroblastoma cell-based impedance biosensor.
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DOI：
10.1016/j.bios.2014.09.005
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发表时间：
2015-05
影响因子：
12.6
通讯作者：
L. Zou;Chunsheng Wu;Qin Wang;Jie Zhou;Kaiqi Su;Hongbo Li;N. Hu;Ping Wang
中科院分区：
文献类型：
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作者：
L. Zou;Chunsheng Wu;Qin Wang;Jie Zhou;Kaiqi Su;Hongbo Li;N. Hu;Ping Wang
麻痹性贝类毒素（Paralytic shellfish poisoning，PSP）是一种钠离子通道阻断性海洋毒素，可阻断神经冲动的传导，导致一系列神经系统疾病症状。然而，PSP毒素可以抑制化合物的细胞毒性作用（例如，哇巴因和藜芦定）。在哇巴因和藜芦碱作用下，神经母细胞瘤细胞逐渐肿胀和死亡，这是由于藜芦碱引起Na+持续内流，而哇巴因抑制Na+/K+-ATP酶活性。因此，具有拮抗作用的PSP毒素可以通过阻断Na+的流入来提高细胞存活的机会。基于PSP毒素的拮抗作用，我们设计了一种基于神经母细胞瘤细胞的阻抗生物传感器检测PSP毒素的改进的基于细胞的检测方法。结果表明，该生物传感器对蛤蚌毒素的检测具有较高的灵敏度和良好的特异性。该生物传感器的检测限低至0.03 ng/ml，低于先前报道的基于细胞的测定和小鼠生物测定。随着生物传感器性能的不断提高，神经母细胞瘤细胞阻抗生物传感器有望成为一种通用的PSP筛选方法。
Paralytic shellfish poisoning (PSP) toxins are well-known sodium channel-blocking marine toxins, which block the conduction of nerve impulses and lead to a series of neurological disorders symptoms. However, PSP toxins can inhibit the cytotoxicity effect of compounds (e.g., ouabain and veratridine). Under the treatment of ouabain and veratridine, neuroblastoma cell will swell and die gradually, since veratridine causes the persistent inflow of Na+and ouabain inhibits the activity of Na+/K+-ATPases. Therefore, PSP toxins with antagonism effect can raise the chance of cell survival by blocking inflow of Na+. Based on the antagonism effect of PSP toxins, we designed an improved cell-based assay to detect PSP toxins using a neuroblastoma cell-based impedance biosensor. The results demonstrated that this biosensor showed high sensitivity and good specificity for saxitoxins detection. The detection limit of this biosensor was as low as 0.03 ng/ml, which was lower than previous reported cell-based assays and mouse bioassays. With the improvement of biosensor performance, the neuroblastoma cell-based impedance biosensor has great potential to be a universal PSP screening method.