Insights into the translational regulation of biologically active open reading frames of Pelargonium line pattern virus
Insights into the translational regulation of biologically active open reading frames of Pelargonium line pattern virus
复制标题
DOI：
10.1016/j.virol.2009.01.017
复制
发表时间：
2009-04-10
期刊：
影响因子：
3.7
通讯作者：
Hernandez, Carmen
中科院分区：
文献类型：
--
作者：
Castano, Aurora;Ruiz, Leticia;Hernandez, Carmen
天竺葵线型病毒(PLPV)是番茄病毒科PelarspoVirus属的一个新成员，其基因组RNA为单链正链。根据先前的预测，它含有6个开放阅读框(ORF)，可能编码27(P27)、13(P13)、87(P87)、7(P7)、6(P6)和37 kDa(P37)的蛋白质。使用各种技术，我们证明了除了(P13)和(P6)之外，所有预测的ORF都是功能的。我们还鉴定了一个先前未知的ORF，它编码9.7 kDa的蛋白质(p9.7)，这是生命运动所必需的。此外，我们提出的证据表明，病毒产生的单个亚基因组RNA(SgRNA)指导p7、p9.7和p37的合成。值得注意的是，这些完全无关的蛋白质的翻译是通过泄漏扫描来协调的。ORF的起始密码子(P7)、ORF的非AUG弱起始密码子(P9.7)以及ORF(P37)之前的任何阅读框架中缺乏额外的AUG密码子的翻译上下文似乎有利于这种机制。研究结果还表明，对sgRNA蛋白生产的精确调控对病毒的生存至关重要。总之，这些数据支持PLPV属于一种新的植物病毒属的观点。(C)2009 Elsevier Inc.保留所有权利
Pelargonium line pattern virus (PLPV), a proposed member of a prospective genus (Pelarspovirus) within family Tombusviridae, has a positive-sense, single-stranded genomic RNA. According to previous predictions, it contains six open reading frames (ORFs) potentially encoding proteins of 27 (p27), 13 (p13) 87 (p87) 7 (p7), 6 (p6), and 37 kDa (p37). Using a variety of techniques we demonstrate that all predicted ORFs are functional, with the exception of (p13) and (p6). We also characterize a previously unidentified ORF which encodes a 9.7 kDa protein (p9.7) that is essential for vital movement. Furthermore, we present evidence that the single subgenomic RNA (sgRNA) produced by the virus directs synthesis of p7, p9.7 and p37. Remarkably, the translation of these totally unrelated proteins is coordinated via leaky-scanning. This mechanism seems to be favoured by the poor translation context of the start codon of ORF(p7), the non-AUG weak initiation codon of ORF(p9.7) and the lack of additional AUG codons in any reading frame preceding ORF(p37). The results also suggest that precise regulation of protein production from the sgRNA is critical for Virus viability. Altogether, the data supports the notion that PLPV belongs to a new genus of plant Viruses. (C) 2009 Elsevier Inc. All rights reserved