Protein secretion systems in Fusobacterium nucleatum:: Genomic identification of Type 4 piliation and complete Type V pathways brings new insight into mechanisms of pathogenesis
Protein secretion systems in Fusobacterium nucleatum:: Genomic identification of Type 4 piliation and complete Type V pathways brings new insight into mechanisms of pathogenesis
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DOI：
10.1016/j.bbamem.2005.05.002
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发表时间：
2005-07-30
影响因子：
3.4
通讯作者：
Henderson, IR
中科院分区：
文献类型：
--
作者：
Desvaux, M;Khan, A;Henderson, IR
最近的基因组分析的两个测序菌株F nucleatum subsp.具核镰孢菌ATCC 25586和具核镰孢菌亚种（F nucleatum subsp.）vincentii ATCC 49256表明主要的蛋白分泌系统不存在。然而，这种缺乏的蛋白分泌系统是不协调的F核的发病机制。此外，对于迄今为止测序的每种其他革兰氏阴性生物体，已经描述了一种或多种这样的系统的存在。在这项调查中，蛋白质分泌的问题，在F核被重新审视。在目前的研究中，F.双精氨酸易位系统的核质（TC #2.A.64.），III型分泌系统（TC #3.A.6.），a IV型分泌系统（TC #3.A.7.）和分子伴侣/引导途径（TC #1.13.11.）得到了证实。然而，与以前的研究结果相反，我们的研究表明，I型蛋白分泌系统也不存在于F核。相比之下，holin家族（TC #1.E）的成员和4型纤毛/菌毛系统（TC #3.A.15.2.）使用各种生物信息学工具进行鉴定。此外，类似于V型分泌途径成员的蛋白质的完整范围，即，Va型（自动运输机; TC #1.B.12.），Vb型（双体分泌系统; TIC #1.B.20.）和Vc型（YadA样三聚体自身转运蛋白; TIC#1.B.42.），本研究为深入了解具核镰刀菌的蛋白分泌和毒力机制提供了新的思路。(c)2005 Elsevier B. V.保留所有权利。
Recent genomic analyses of the two sequenced strains F nucleatum subsp. nucleatum ATCC 25586 and F nucleatum subsp. vincentii ATCC 49256 suggested that the major protein secretion systems were absent. However, such a paucity of protein secretion systems is incongruous with F nucleatum pathogenesis. Moreover, the presence of one or more such systems has been described for every other Gram-negative organism sequenced to date. In this investigation, the question of protein secretion in F nucleatum was revisited. In the current study, the absence in F. nucleatum of a twin-arginine translocation system (TC #2.A.64.), a Type III secretion system (TC #3.A.6.), a Type IV secretion system (TC #3.A.7.) and a chaperone/usher pathway (TC #1.13.11.) was confirmed. However, contrary to previous findings, our investigations indicated that a Type I protein secretion system was also absent from F nucleatum. In contrast, members of the holin family (TC #1.E) and the machinery required for a Type 4 piliation/fimbriation system (TC #3.A.15.2.) were identified using a variety of bioinformatic tools. Furthermore, a complete range of proteins resembling members of the Type V secretion pathway, i.e., the Type Va (autotransporter; TC #1.B.12.), Type Vb (two-partner secretion system; TIC #1.B.20.) and Type Vc (YadA-like trimeric autotransporter; TIC #1.B.42.), was found. This work provides new insight into the protein secretion and virulence mechanisms of F nucleatum. (c) 2005 Elsevier B.V. All rights reserved.