Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines
Alteration of antioxidant enzyme gene expression during cold acclimation of near-isogenic wheat lines
复制标题
DOI：
10.1016/s0168-9452(03)00329-7
复制
发表时间：
2003-12-01
期刊：
影响因子：
5.2
通讯作者：
Skinner, DZ
中科院分区：
文献类型：
--
作者：
Baek, KH;Skinner, DZ
由于膜、DNA、蛋白质和碳水化合物的潜在氧化，活性氧物种(ROS)对生物体有害。冻伤已被证明与ROS的攻击有关。抗氧化酶可以保护植物细胞免受冰冻伤害造成的氧化胁迫，因此，冷驯化可能涉及抗氧化酶表达的增加。本研究利用定量RT-PCR技术检测冬小麦和春小麦近等基因系(NILs)在冷驯化过程中抗氧化酶的表达水平，这些NILs具有不同的Vrn1-Fr1染色体区域。检测到的抗氧化基因有线粒体锰超氧化物歧化酶(MnSOD)、叶绿体铜锌超氧化物歧化酶(铜、锌超氧化物歧化酶)、铁超氧化物歧化酶(FeSOD)、过氧化氢酶(CAT)、类囊体结合抗坏血酸过氧化物酶(t-APX)、胞质谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GPx)、胞质单脱氢抗坏血酸还原酶(MDAR)、叶绿体脱氢抗坏血酸还原酶(DHAR)。表达上调(MnSOD、MDAR、t-APX、Dhar、GPX和GR)，下调(CAT)或相对恒定(FeSOD和铜、锌超氧化物歧化酶)。冷驯化4周后，冬小麦近等基因系中t-APX、CAT和MnSOD的表达水平显著高于春小麦近等基因系，因此VRN-Fr1区似乎对某些抗氧化酶基因的表达具有调节作用。(C)2003爱思唯尔爱尔兰有限公司。保留所有权利。
Reactive oxygen species (ROS) are harmful to living organisms due to the potential oxidation of membranes, DNA, proteins, and carbohydrates. Freezing injury has been shown to involve the attack of ROS. Antioxidant enzymes can protect plant cells from oxidative stress imposed by freezing injury; therefore, cold acclimation may involve an increase in the expression of antioxidant enzymes. In this study, quantitative RT-PCR was used to measure the expression levels of antioxidant enzymes during cold acclimation in near-isogenic lines (NILs) of wheat, differing in the Vrn1-Fr1 chromosome region that conditions winter versus spring wheat growth habit. The antioxidant genes monitored were mitochondrial manganese superoxide dismutase (MnSOD), chloroplastic Cu,Zn superoxide dismutase (Cu,ZnSOD), iron superoxide dismulase (FeSOD), catalase (CAT), thylakoid-bound ascorbate peroxidase (t-APX), cytosolic glutathione reductase (GR), glutathione peroxidase (GPX), cytosolic mono-dehydroascorbate reductase (MDAR), chloroplastic dehydroascorbate reductase (DHAR). The expression levels were upregulated (MnSOD, MDAR, t-APX, DHAR, GPX, and GR), downregulated (CAT), or relatively constant (FeSOD and Cu,ZnSOD). The Vrn-Fr1 region seemed to have a role in regulating the expression level of some of the antioxidant enzyme genes because t-APX, CAT and MnSOD expressed to significantly higher levels in the winter wheat NIL than the spring wheat NIL after 4 weeks' cold acclimation. (C) 2003 Elsevier Ireland Ltd. All rights reserved.