ASYMMETRIC SEGREGATION OF NUMB AND PROSPERO DURING CELL-DIVISION
ASYMMETRIC SEGREGATION OF NUMB AND PROSPERO DURING CELL-DIVISION
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DOI：
10.1038/377624a0
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发表时间：
1995-10-19
期刊：
影响因子：
64.8
通讯作者：
JAN, YN
中科院分区：
文献类型：
--
作者：
KNOBLICH, JA;JAN, LY;JAN, YN
一个细胞可以通过将一个决定簇特异性地分离到它的一个子细胞中来进行不对称分裂（1）。Numb蛋白是发育中的果蝇神经系统的不对称细胞分裂中这种决定子的候选者（2，3）。Numb是一种膜相关蛋白，其在细胞分裂期间不对称定位并分离成一个子细胞，在子细胞中需要它来指定正确的细胞命运（3-5）。在这里，我们表明，核蛋白，Prospero（6，7），易位到膜在细胞分裂的开始和共定位与Numb在整个有丝分裂，这表明一个共同的机制，不对称分离。Numb和Prospero定位与有丝分裂偶联，并与两个中心体之一的位置密切相关。然而，与中心体定位相反，Numb和Prospero定位独立于微管。细胞松弛素D治疗表明，该过程也是独立的肌动蛋白，我们建议，有一个组织者的不对称细胞分裂，提供了位置信息的方向的有丝分裂纺锤体和不对称本地化的Numb和Prospero。
A CELL can divide asymmetrically by specifically segregating a determinant into one of its daughter cells(1). The Numb protein is a candidate for such a determinant in the asymmetric cell divisions of the developing Drosophila nervous system(2,3). Numb is a membrane-associated protein that localizes asymmetrically during cell division and segregates into one daughter cell, where it is required for the specification of the correct cell fate(3-5). Here we show that a nuclear protein, Prospero(6,7), translocates to the membrane at the beginning of cell division and colocalizes with Numb throughout mitosis, suggesting a common mechanism for asymmetric segregation. Numb and Prospero localization is coupled to mitosis and tightly correlated with the position of one of the two centrosomes. In contrast to centrosome positioning, however, Numb and Prospero localization is independent of microtubules. Cytochalasin D treatment suggests that the process is also independent of actin, We propose that there is an organizer of asymmetric cell division which provides positional information for both the orientation of the mitotic spindle and asymmetric localization of Numb and Prospero.