A population of prenatally generated cells in the rat paleocortex maintains an immature neuronal phenotype into adulthood
A population of prenatally generated cells in the rat paleocortex maintains an immature neuronal phenotype into adulthood
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DOI：
10.1093/cercor/bhm255
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发表时间：
2008-10-01
期刊：
影响因子：
3.7
通讯作者：
Nacher, Juan
中科院分区：
文献类型：
--
作者：
Angeles Gomez-Climent, Maria;Castillo-Gomez, Esther;Nacher, Juan
成人大脑中的新神经元瞬间表达与神经元发育相关的分子，如神经细胞粘附分子的多唾液化形式，或双皮质素（DCX）。这些分子也在大鼠皮层第二层的细胞群中表达，其起源、表型和功能尚不清楚。我们将这些细胞中的大多数归类为一种新的细胞类型，称为缠结细胞。在这个群体中还发现了一些具有半月-锥体过渡性神经元形态的细胞，以及一些罕见的类似半月、锥体的细胞。梭状神经元。我们发现这些细胞在第II层中没有表达胶质细胞、成熟细胞、抑制性细胞或主要神经元的标记物。DCX、TOAD/Ulip/CRMP-4、环核苷酸门控通道的A3亚基或磷酸化环腺苷单磷酸反应元件结合蛋白的共表达证实了它们似乎处于长时间的未成熟状态。此外，它们大多缺乏突触接触，被星形胶质片覆盖，不能表达细胞活性标记物，如c-Fos或Arc，以及n -甲基-d-天冬氨酸或糖皮质激素受体。我们发现，这些细胞似乎都不是在成年期或青年期早期产生的，大多数是在胚胎发育期间产生的，主要是在E15.5。
New neurons in the adult brain transiently express molecules related to neuronal development, such as the polysialylated form of neural cell adhesion molecule, or doublecortin (DCX). These molecules are also expressed by a cell population in the rat paleocortex layer II, whose origin, phenotype, and function are not clearly understood. We have classified most of these cells as a new cell type termed tangled cell. Some cells with the morphology of semilunar-pyramidal transitional neurons were also found among this population, as well as some scarce cells resembling semilunar, pyramidal. and fusiform neurons. We have found that none of these cells in layer II express markers of glial cells, mature, inhibitory, or principal neurons. They appear to be in a prolonged immature state, confirmed by the coexpression of DCX, TOAD/Ulip/CRMP-4, A3 subunit of the cyclic nucleotide-gated channel, or phosphorylated cyclic adenosine monophosphate response element-binding protein. Moreover, most of them lack synaptic contacts, are covered by astroglial lamellae, and fail to express cellular activity markers, such as c-Fos or Arc, and N-methyl-d-aspartate or glucocorticoid receptors. We have found that none of these cells appear to be generated during adulthood or early youth and that most of them have been generated during embryonic development, mainly in E15.5.