Sources and spread of thermophilic Campylobacter spp. during partial depopulation of broiler chicken flocks
Sources and spread of thermophilic Campylobacter spp. during partial depopulation of broiler chicken flocks
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DOI：
10.4315/0362-028x-71.2.264
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发表时间：
2008-02-01
影响因子：
2
通讯作者：
Edge, S.
中科院分区：
文献类型：
--
作者：
Allen, V. M.;Weaver, H.;Edge, S.
由于在间伐过程中难以维持生物安全，部分人口减少或间伐(提前从商业肉鸡群中移走一部分禽类)的做法被报告为弯曲杆菌对剩余禽类进行定植的风险因素。对51个目标鸡群的这种做法的效果进行了详细的研究，每个鸡群都在一个不同的养殖场，属于联合王国七家主要家禽公司之一。在其中21个养殖场，目标鸡群已被弯曲杆菌定植，屠宰时所有盲肠样本均为阳性，平均为8logCFU/g。另有27只鸡群在开始间伐后2-6天内呈阳性，屠宰时盲肠携带水平同样高。就在间伐过程之前，经常从农场车道、运输车辆、设备和人员中分离出弯曲杆菌。采用脉冲场凝胶电泳法对间伐后定植的7个养殖场的菌株进行了检测。在特定采样点出现的菌株与随后从稀疏鸡群中分离的菌株之间发现了关联。结果表明，当农场由同一公司共同拥有并使用相同的捕鸟队和/或车辆时，特定菌株已从一个农场传播到另一个农场。这些结果突出表明，需要对捕获设备和人员进行更好的卫生控制，并对车辆和鸟类运输箱进行更有效的清洁和消毒。
The practice of partial depopulation or thinning (early removal of a portion of birds from a commercial broiler flock) is a reported risk factor for Campylobacter colonization of residual birds because of the difficulty in maintaining biosecurity during the thinning process. The effect of this practice was studied in detail for 51 target flocks, each at a different growing farm belonging to one of seven major poultry companies throughout the United Kingdom. On 21 of these farms, the target flock was already colonized by Campylobacter, and at slaughter all cecal samples examined were positive, with a mean of 8 log CFU/g. An additional 27 flocks became positive within 2 to 6 days of the start of thinning and had similarly high levels of cecal carriage at slaughter. Just before the thinning process, Campylobacter was isolated frequently from the farm driveways, transport vehicles, equipment, and personnel. Strains from seven farms on which flocks became colonized after thinning were examined by pulsed-field gel electrophoresis typing. An association was found between strains occurring at specific sampling sites and those isolated subsequently from the thinned flocks. The results indicated that particular strains had spread from one farm to another when the farms were jointly owned by the same company and employed the same bird-catching teams and/or vehicles. These results highlight the need for better hygiene control in relation to catching equipment and personnel and more effective cleaning and disinfection of vehicles and bird-transport crates.