Behaviour of a Temperature Sensitive Strain of Tobacco Mosaic Virus in Tomato Leaves and Protoplasts
Behaviour of a Temperature Sensitive Strain of Tobacco Mosaic Virus in Tomato Leaves and Protoplasts
复制标题
烟草花叶病毒温度敏感株在番茄叶和原生质体中的行为
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
N. Oshima
中科院分区：
文献类型：
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作者：
M. Nishiguchi;F. Motoyoshi;N. Oshima
总结
从烟草花叶病毒（TMV）番茄株系L的培养物中分离到一个温度敏感株系Ls 1。Ls 1在过敏烟草叶片上引起的坏死局部病变比L小。温度转换处理（22 °C持续3天，32 °C持续2天和22 °C持续1天）允许L产生被塌陷区域包围的坏死病变，而Ls 1引起坏死病变而没有塌陷周围区域，这表明Ls 1在32 °C下不会扩散到病变外部。在22 °C的番茄叶圆片中，Ls 1的感染性与L的感染性平行增加，但在32 °C下，Ls 1的感染性增加可以忽略不计，与L的感染性的大幅增加形成对比。来自在32 °C下孵育的Lsl接种的盘的少数叶肉细胞被荧光抗体染色，但在22 °C下孵育的许多叶肉细胞被染色。然而，在32 °C的原生质体中，Ls 1和L感染并繁殖相似。将接种Ls 1的叶片在20 ~ 25 ℃下放置24 h，然后制备圆片，在32 ℃或22 ℃下培养12 h，32 ℃下含病毒抗原的叶肉细胞比例没有增加，但感染性大大增加。这表明Ls 1在32 °C下在已经感染的细胞中容易繁殖。在22 °C下，感染细胞的比例和叶提取物的感染性迅速增加。在32 °C下，Ls 1在叶圆片中的生长曲线类似于Ls 1或L在原生质体中的所谓“一步生长曲线”，而不是L在圆片中的生长曲线，其中病毒可以从细胞传播到细胞。这些结果表明，Ls 1是一个ts菌株，在非允许的温度下正常增殖，但在细胞间的运动有故障。
Summary
A temperature-sensitive (ts) strain, Ls1, was isolated from a culture of L, a tomato strain of tobacco mosaic virus (TMV). Ls1 caused smaller necrotic local lesions than L in leaves of hypersensitive tobacco plants. A temperature shift treatment (22 °C for 3 days, 32 °C for 2 days and 22 °C for 1 day) allowed L to produce necrotic lesions surrounded by a collapsed area, whereas Ls1 caused necrotic lesions without a collapsed surrounding area, suggesting that Ls1 did not spread outside the lesions at 32 °C. In tomato leaf discs at 22 °C, infectivity of Ls1 increased in parallel with that of L, but at 32 °C the increase in infectivity of Ls1 was negligible and contrasted with the large increase of infectivity of L. Few mesophyll cells from Ls1-inoculated discs incubated at 32 °C were stained by fluorescent antibody but many of those incubated at 22 °C were stained. In protoplasts at 32 °C, however, Ls1 and L infected and multiplied similarly. When leaves inoculated with Ls1 were kept at 20 to 25 °C for 24 h, and discs were then prepared and cultured for 12 h at 32 or 22 °C, the proportion of mesophyll cells containing virus antigen did not increase at 32 °C but infectivity increased greatly. This suggests that Ls1 multiplied readily at 32 °C in already infected cells. At 22 °C the proportion of infected cells and the infectivity of leaf extracts increased rapidly. The growth curve of Ls1 in leaf discs at 32 °C resembled the so-called ‘one-step growth curve’ of Ls1 or L in protoplasts, and not the growth curve of L in discs, in which the virus could spread from cell to cell. These results suggest that Ls1 is a ts strain that multiplies normally at the non-permissive temperature but has a malfunction in cell-to-cell movement.