Accurate quantitation of the low number of white cells in white cell-depleted blood components.
Accurate quantitation of the low number of white cells in white cell-depleted blood components.
复制标题
对去除白细胞的血液成分中的少量白细胞进行准确定量。
DOI：
10.1046/j.1537-2995.1989.29990070180.x
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Scornik,JC
中科院分区：
文献类型：
--
作者：
Kao,KJ;Scornik,JC
利用碘化丙啶（PI）对白细胞核进行染色，建立了一种简便、准确的测定白细胞（WBC）浓度的方法。通过将PI测定的白细胞浓度与库尔特S - plus IV计数器测定的白细胞浓度（红细胞或随机供体血小板）进行比较，验证了该方法的有效性。相关系数为0.99，呈线性关系。PI染色的灵敏度允许检测低至每μL rdp 1个细胞或每μL红细胞11个细胞的白细胞浓度。因此，PI染色将有助于研究输注白细胞耗尽的血液成分在预防HLA抗原的初次同种免疫中的作用，以及评估各种高效地从血液成分中消耗白细胞的新方法。
A simple method of accurate quantification of low concentrations of white cells (WBCs) in WBC‐depleted blood components was developed by using propidium iodide (PI) to stain the nuclei of WBCs. The method was validated by correlating the PI‐determined WBC concentrations with those determined with Coulter S‐plus IV counter in units of packed red cells (PRBCs) or random‐donor platelets (RDPs). The correlations were linear and had coefficients of 0.99. The sensitivity of PI staining permitted the detection of concentrations of WBCs as low as 1 cell per μL of RDPs or 11 cells per μL of PRBCs. Therefore, PI staining will be useful in investigating the role of transfused WBC‐depleted blood components in the prevention of primary alloimmunization to HLA antigens, as well in evaluating various new procedures with high efficiency in depleting WBCs from blood components.