Effect of age on cerebral venous circulation disturbances in the rat.
Effect of age on cerebral venous circulation disturbances in the rat.
复制标题
年龄对大鼠脑静脉循环障碍的影响。
DOI：
10.3171/jns.2000.93.2.0298
复制
发表时间：
2000
影响因子：
4.1
通讯作者：
Toshisuke Sakaki
中科院分区：
文献类型：
--
作者：
H. Otsuka;Hiroyuki Nakase;Kiyoshi Nagata;Katsuhiro Ueda;Oliver Kempski;Toshisuke Sakaki
对象
在神经外科实践中，老年患者的轻度脑静脉循环障碍（CVCD）经常导致意外严重的术后并发症。本研究的目的是确定是否有年龄相关的差异参与CVCD的脆弱性。
方法
使用了38只雄性Wistar大鼠。在年轻（Y组，19只10-14周龄的动物）和老年（A组，7只80-100周龄的动物）大鼠中，通过使用玫瑰红染料和光纤照明，以光化学方式闭塞直径为100 μ m的单个皮质静脉。5只幼龄和7只老龄动物作为假手术对照。局部脑血流量（rCBF）由局部CBF确定，局部CBF在25（5 x 5）个相同位置测量，闭塞静脉位于扫描场中心，静脉闭塞后每15分钟使用激光多普勒扫描技术持续90分钟。采用荧光血管造影术检查脑静脉血流模式，直至闭塞后90分钟。在闭塞后24小时检查组织学标本。Y组静脉阻断后rCBF无明显变化。而A组在闭塞后15分钟开始rCBF迅速下降。在闭塞后30、60和90分钟观察到显著的组间差异。静脉血流停止，导致静脉梗死，血管造影观察到闭塞后90分钟，在两个（10.5%）的19个年轻和六个（85.7%）的7个老年大鼠。栓塞后90 min，A组大鼠静脉血栓明显大于Y组大鼠。在所有老年大鼠（100%）和6只年轻大鼠（31.6%）中均观察到静脉梗死;老年大鼠的梗死面积（表示为同侧半球面积的百分比）明显大于年轻大鼠。
结论
这项研究表明，静脉闭塞后静脉梗死的发生率和大小与年龄相关，这表明老年大脑中更容易发生CVCD，这可能是由于闭塞静脉引流的有限脑区早期和广泛的灌注不足。老年动物中更大的血栓形成表明血栓形成/血栓溶解平衡的转变是观察到的效果的原因。
OBJECT
Mild cerebral venous circulation disturbances (CVCDs) in aged patients are frequently known to cause unexpectedly severe postoperative complications in neurosurgical practice. The object of the present study was to determine whether there are age-related differences involved in vulnerability to CVCDs.
METHODS
Thirty-eight male Wistar rats were used. A single cortical vein with a 100-microm diameter was occluded photochemically by using rose bengal dye and fiberoptic illumination in young (Group Y, 19 animals aged 10-14 weeks) and aged (Group A, seven animals aged 80-100 weeks) rats. Five young and seven aged animals served as sham-operated controls. Regional cerebral blood flow (rCBF) was determined from local CBF, which was measured at 25 (5 x 5) identical locations, with the occluded vein located central to the scanning field, by using a laser Doppler scanning technique every 15 minutes for 90 minutes after venous occlusion. The cerebral venous flow pattern was examined using fluorescence angiography until 90 minutes after occlusion. Histological specimens were examined 24 hours after occlusion. In Group Y, rCBF did not change significantly after venous occlusion. However, in Group A, rCBF decreased rapidly beginning 15 minutes after occlusion. Significant intergroup differences were observed 30, 60, and 90 minutes after occlusion. Venous flow arrest, which resulted in venous infarct, was observed on angiography 90 minutes after occlusion in two (10.5%) of 19 young and six (85.7%) of seven aged rats. The venous thrombus in Group A rats was significantly larger than that in Group Y rats 90 minutes after occlusion. Venous infarction was seen in all aged rats (100%) and in six young rats (31.6%); the infarct size, expressed as a percentage of the size of the ipsilateral hemisphere, was significantly larger in aged rats than in young rats.
CONCLUSIONS
This study demonstrated an age-related increase in the rate and size of venous infarct following vein occlusion, suggesting that the greater vulnerability to CVCDs in the aged brain might be attributed to early and extensive hypoperfusion of circumscribed brain areas drained by the occluded vein. The larger thrombus formation in aged animals indicates that a shift in the thrombogenetic/thrombolytic equilibrium is responsible for the observed effect.
衰老引起的脑氧化代谢异常及其与中枢去甲肾上腺素能系统的关系。
影响因子：
3.5
作者：
Sylvia,AL;Harik,SI;LaManna,JC;Wilkerson,T;Rosenthal,M
通讯作者：
Rosenthal,M
大鼠大脑中神经生长因子的发育表现：老年前脑的显着缺陷。
影响因子：
--
作者：
Lärkfors,L;Ebendal,T;Whittemore,SR;Persson,H;Hoffer,B;Olson,L
通讯作者：
Olson,L
哺乳动物脑神经元的神经营养因子：新生儿、成年和老年大鼠脑损伤诱导。
影响因子：
2.9
作者：
Whittemore,SR;Nieto-Sampedro,M;Needels,DL;Cotman,CW
通讯作者：
Cotman,CW