Identification of bladder wall layers by Raman spectroscopy
Identification of bladder wall layers by Raman spectroscopy
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DOI：
10.1016/s0022-5347(05)64411-4
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发表时间：
2002-10-01
影响因子：
6.6
通讯作者：
Puppels, GJ
中科院分区：
文献类型：
--
作者：
De Jong, BWD;Schut, TCB;Puppels, GJ
用途：我们探讨了拉曼光谱原位调查膀胱壁tissue.Materials和方法：膀胱壁组织从豚鼠模型和冰冻切片被用于拉曼光谱investigations。从每个部分500至700光谱中获得的2维网格跨越尿道，固有层和肌肉层。通过聚类分析算法将光谱数据集细分为相似光谱组。每组分配不同的颜色拉曼地图的冷冻切片的基础上组成员的测量光谱。这些地图进行比较，从苏木精和伊红和免疫组化染色的胶原蛋白I和III和平滑肌肌动蛋白相关拉曼光谱特征与膀胱壁的结构和分子composition.Results：尿路上皮，固有层和肌肉层可以清楚地区分拉曼光谱的基础上获得的组织学和组织化学数据。固有层光谱主要由胶原蛋白的信号贡献和平滑肌层表现出强烈的信号贡献肌动蛋白。urothelium有一个相对较强的脂质信号contribution.Conclusions：这些结果和事实，即拉曼光谱正在迅速发展成为一种技术，可以应用在体内薄，灵活的光纤导管表明，前景是良好的正常和病理膀胱的分子组成的活体分析，而无需活检。
Purpose: We explored the applicability of Raman spectroscopy to in situ investigation of bladder wall tissue.Materials and Methods: Bladder wall tissue was obtained from a guinea pig model and frozen sections were used for Raman spectroscopic investigations. From each section 500 to 700 spectra were obtained in a 2-dimensional grid spanning the urothelium, lamina propria and muscle layer. The data set of spectra was subdivided into groups of similar spectra by a cluster analysis algorithm. With each group assigned a different color Raman maps of frozen sections were constructed based on group membership of measured spectra. These maps were then compared with histological and histochemical data obtained from hematoxylin and eosin and immunohistochemical staining for collagen I and III and for smooth muscle actin to correlate Raman spectral features with bladder wall structure and molecular composition.Results: Urothelium, lamina propria and muscle layers could be clearly distinguished based on Raman spectra. Lamina propria spectra were dominated by signal contributions of collagen and the smooth muscle layer showed strong signal contributions of actin. The urothelium had a relatively strong lipid signal contribution.Conclusions: These results and the fact that Raman spectroscopy is rapidly evolving into a technology that can be applied in vivo by thin, flexible fiberoptic catheters indicate that prospects are good for in vivo analysis of the molecular composition of the normal and pathological bladder without biopsies.