AN NSF-LIKE ATPASE, P97, AND NSF MEDIATE CISTERNAL REGROWTH FROM MITOTIC GOLGI FRAGMENTS
AN NSF-LIKE ATPASE, P97, AND NSF MEDIATE CISTERNAL REGROWTH FROM MITOTIC GOLGI FRAGMENTS
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DOI：
10.1016/0092-8674(95)90270-8
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发表时间：
1995-09-22
期刊：
影响因子：
64.5
通讯作者：
WARREN, G
中科院分区：
文献类型：
--
作者：
RABOUILLE, C;LEVINE, TP;WARREN, G
在无细胞系统中，高尔基体池从单独用缓冲液孵育的有丝分裂高尔基体片段重新生长。NEM或盐洗预处理可抑制再生长，但这可以通过p97（一种NSF样ATP酶）或NSF与SNAP和p115（一种囊泡对接蛋白）一起恢复。与p97和NSF-SNAPs-p115再生的池的形态不同，这表明它们在有丝分裂后重建高尔基池中发挥不同的作用。
Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.