Interactions of PLGA nanoparticles with blood components: protein adsorption, coagulation, activation of the complement system and hemolysis studies
Interactions of PLGA nanoparticles with blood components: protein adsorption, coagulation, activation of the complement system and hemolysis studies
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DOI：
10.1039/c5nr00733j
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发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Vauthier, Christine
中科院分区：
文献类型：
--
作者：
Fornaguera, Cristina;Caldero, Gabriela;Vauthier, Christine
聚（乳酸-羟基乙酸）（PLGA）纳米颗粒的静脉给药已被广泛报道为一种有前途的替代药物输送到特定的细胞。然而，使用不同的技术，他们与不同的血液成分的相互作用的研究仍然缺乏。因此，在目前的工作中，PLGA纳米粒子与血液成分的相互作用，使用不同的互补技术进行了描述。还报道了不同包封化合物/官能化试剂对这些相互作用的影响。值得注意的是，所有这些技术都可以简单地执行，而不需要高度复杂的设备或技能。此外，它们可以很容易地转移到工业和质量控制的应用。血清白蛋白被吸附到所有类型的测试纳米颗粒上。饱和浓度取决于纳米颗粒的尺寸。相反，纤维蛋白原聚集依赖于纳米颗粒表面电荷。补体激活也受到纳米颗粒功能化的影响;功能化试剂的存在增加了补体激活，而添加包封的化合物仅引起轻微增加。在低浓度下，没有纳米颗粒影响凝血级联反应。然而，在高浓度下，阳离子化纳米颗粒确实激活了凝血级联反应。纳米颗粒与红细胞的相互作用没有显示任何溶血。PLGA纳米颗粒与血液蛋白的相互作用取决于纳米颗粒的性质和研究的蛋白质。独立于其负载/表面功能化，PLGA纳米颗粒不影响凝血级联反应，也不诱导红细胞溶血;在诱导栓塞和细胞溶解方面，可以将其定义为安全的。
The intravenous administration of poly(lactic-co-glycolic) acid (PLGA) nanoparticles has been widely reported as a promising alternative for delivery of drugs to specific cells. However, studies on their interaction with diverse blood components using different techniques are still lacking. Therefore, in the present work, the interaction of PLGA nanoparticles with blood components was described using different complementary techniques. The influence of different encapsulated compounds/functionalizing agents on these interactions was also reported. It is worth noting that all these techniques can be simply performed, without the need for highly sophisticated apparatus or skills. Moreover, their transference to industries and application of quality control could be easily performed. Serum albumin was adsorbed onto all types of tested nanoparticles. The saturation concentration was dependent on the nanoparticle size. In contrast, fibrinogen aggregation was dependent on nanoparticle surface charge. The complement activation was also influenced by the nanoparticle functionalization; the presence of a functionalizing agent increased complement activation, while the addition of an encapsulated compound only caused a slight increase. None of the nanoparticles influenced the coagulation cascade at low concentrations. However, at high concentrations, cationized nanoparticles did activate the coagulation cascade. Interactions of nanoparticles with erythrocytes did not reveal any hemolysis. Interactions of PLGA nanoparticles with blood proteins depended both on the nanoparticle properties and the protein studied. Independent of their loading/surface functionalization, PLGA nanoparticles did not influence the coagulation cascade and did not induce hemolysis of erythrocytes; they could be defined as safe concerning induction of embolization and cell lysis.