Characterization of 298 ESTs from porcine back fat tissue and their assignment to the SSRH radiation hybrid map

Characterization of 298 ESTs from porcine back fat tissue and their assignment to the SSRH radiation hybrid map
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DOI:
10.1007/s00335-003-2323-7
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发表时间:
2004-04-01
期刊:
影响因子:
2.5
通讯作者:
Hamasima, N
Hamasima, N
中科院分区:
生物学4区
文献类型:
--
作者:
Mikawa, A;Suzuki, H;Hamasima, N

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根据几个标准,我们从迄今为止收集的超过 15,000 个猪背膘文库中收集、聚类和功能分类 653 个 5' 端表达序列标签 (EST)。通过搜索LocusLink和Mapviewer数据库,我们知道了这653个EST在人类染色体(HSA)上的位置。野猪辐射杂交 (SSRH) 作图显示,653 个猪 EST 簇中的 298 个位于微卫星 (MS) 标记附近。在这些 EST 簇中,我们可以在 SSRH 图谱上将 182 条分配给它们的猪染色体 (SSC)。
Following several criteria, we collected, clustered, and functionally categorized 653 expressed sequence tags (ESTs) of 5' ends from porcine back fat libraries from the >15,000 porcine ESTs collected to date. By searching the LocusLink and Mapviewer database, we knew the positions of these 653 ESTs on human chromosomes (HSAs). Sus scrofa radiation hybrid (SSRH) mapping revealed that 298 porcine EST clusters out of 653 were localized near microsatellite (MS) markers. Among these EST clusters, we could assign 182 to their porcine chromosomes (SSCs) on the SSRH map.