In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat
In vivo 13C NMR assessment of brain glycogen concentration and turnover in the awake rat
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DOI：
10.1016/s0197-0186(03)00018-4
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发表时间：
2003-09-01
影响因子：
4.2
通讯作者：
Gruetter, R
中科院分区：
文献类型：
--
作者：
Choi, IY;Gruetter, R
Brain glycogen metabolism was recently observed in vivo and found to be very slow in the lightly alpha-chloralose anesthetized rat [J. Neurochem. 73 (1999) 1300]. Based on that slow turnover, the total glycogen content in the awake rat brain and its turnover time were assessed after administering C-13-labeled glucose for 48 h. Label incorporation into glycogen, glucose, amino acid, and N-acetyl-aspartate (NAA) resonances was observed. The amount of C-13 label incorporated into glycogen was variable and did not correlate with that in glutamate (r = -0.1, P > 0.86). However, the amount of C-13 label incorporated into glycogen was very similar to that in NAA (r = 0.93), implying similar turnover times between brain glycogen and NAA (similar to10h). Absolute quantification of the total concentration of brain glycogen in the awake, normoglycemic rat yielded 3.3 +/- 0.8 mumol/g (n = 6, mean +/- S.D.). (C) 2003 Elsevier Science Ltd. All rights reserved.
