S100P contributes to promoter demethylation and transcriptional activation of SLC2A5 to promote metastasis in colorectal cancer.
S100P contributes to promoter demethylation and transcriptional activation of SLC2A5 to promote metastasis in colorectal cancer.
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S100 P参与SLC2A5启动子去甲基化和转录激活，促进结直肠癌转移。
DOI：
10.1038/s41416-021-01306-z
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发表时间：
2021-08
影响因子：
8.8
通讯作者：
Deng H
中科院分区：
文献类型：
--
作者：
Lin M;Fang Y;Li Z;Li Y;Feng X;Zhan Y;Xie Y;Liu Y;Liu Z;Li G;Shen Z;Deng H
SLC2A5 is a high-affinity fructose transporter, which is frequently upregulated in multiple human malignant tumours. However, the function and molecular mechanism of SLC2A5 in colorectal cancer (CRC) remain unknown. We detected the expression levels of SLC2A5 in CRC tissues and CRC cell lines by western blotting, qRT-PCR and immunohistochemistry. CRC cell lines with stable overexpression or knockdown of SLC2A5 were constructed to evaluate the functional roles of SLC2A5 in vitro through conventional assays. An intrasplenic inoculation model was established in mice to investigate the effect of SLC2A5 in promoting metastasis in vivo. Methylation mass spectrometry sequencing, methylation specific PCR, bisulphite sequencing PCR, ChIP-qPCR and luciferase reporter assay were performed to investigate the molecular mechanism underlying transcriptional activation of SLC2A5. We found that SLC2A5 was upregulated in colorectal tumour tissues. Functionally, a high level of SLC2A5 expression was associated with increased invasion and metastasis capacities of CRC cells both in vitro and in vivo. Mechanistically, we unveiled that S100P could integrate to a specific region of SLC2A5 promoter, thereby reducing its methylation levels and activating SLC2A5 transcription. Our results reveal a novel mechanism that S100P mediates the promoter demethylation and transcription activation of SLC2A5, thereby promoting the metastasis of CRC.
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影响因子：
3.3
作者：
Koltzscher, M;Neumann, C;Gerke, V
通讯作者：
Gerke, V
影响因子：
11.2
作者：
Ahmad A;Aboukameel A;Kong D;Wang Z;Sethi S;Chen W;Sarkar FH;Raz A
通讯作者：
Raz A
影响因子：
11.2
作者：
Bacci, Marina;Giannoni, Elisa;Morandi, Andrea
通讯作者：
Morandi, Andrea
影响因子：
4.8
作者：
Barone, Sharon;Fussell, Stacey L.;Soleimani, Manoocher
通讯作者：
Soleimani, Manoocher
DOI：
10.1073/pnas.1501605112
发表时间：
2015-08-11
影响因子：
11.1
作者：
Daemen, Anneleen;Peterson, David;Evangelista, Marie
通讯作者：
Evangelista, Marie