A polysaccharide fraction of adlay seed (Coix lachryma-jobi L.) induces apoptosis in human non-small cell lung cancer A549 cells
A polysaccharide fraction of adlay seed (Coix lachryma-jobi L.) induces apoptosis in human non-small cell lung cancer A549 cells
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DOI：
10.1016/j.bbrc.2012.11.058
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发表时间：
2013-01-11
影响因子：
3.1
通讯作者：
Luo, Cheng
中科院分区：
文献类型：
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作者：
Lu, Xiangyi;Liu, Wei;Luo, Cheng
薏苡仁的不同种子提取物在中国已被用于治疗各种癌症，临床数据支持这些提取物用于癌症治疗;然而，其潜在的分子机制尚未得到很好的定义。从C. [医]泪腺变种采用乙醇沉淀法。MTT法检测CP-1诱导A549细胞凋亡的作用，并呈剂量依赖性。扫描电镜下可见凋亡小体。Annexin V-FITC和PI染色分别检测细胞凋亡和S期DNA积累，流式细胞仪检测。CP-1还延长了单细胞凝胶电泳的彗星尾长度，并破坏了线粒体膜电位。Western blotting进一步分析显示caspase-3和caspase-9蛋白表达增加。综上所述，我们的结果表明，CP-1能够抑制A549细胞增殖和诱导凋亡的机制，主要涉及激活的内在线粒体途径。分析数据表明，除了其营养特性之外，CP-1是一种非常有前途的用于开发抗癌药物的候选多糖。(C)2012 Elsevier Inc. All rights reserved.
Different seed extracts from Coix lachryma-jobi (adlay seed) have been used for the treatment of various cancers in China, and clinical data support the use of these extracts for cancer therapy; however, their underlying molecular mechanisms have not been well defined. A polysaccharide fraction, designated as CP-1, was extracted from the C. lachryma-jobi L. var. using the ethanol subsiding method. CP-1 induced apoptosis in A549 cells in a dose-dependent manner, as determined by MTT assay. Apoptotic bodies were observed in the cells by scanning electronic microscopy. Apoptosis and DNA accumulation during S-phase of the cell cycle were determined by annexin V-FITC and PI staining, respectively, and measured by flow cytometry. CP-1 also extended the comet tail length on single cell gel electrophoresis, and disrupted the mitochondrial membrane potential. Further analysis by western blotting showed that the expression of caspase-3 and caspase-9 proteins was increased. Taken together, our results demonstrate that CP-1 is capable of inhibiting A549 cell proliferation and inducing apoptosis via a mechanism primarily involving the activation of the intrinsic mitochondrial pathway. The assay data suggest that in addition to its nutritional properties, CP-1 is a very promising candidate polysaccharide for the development of anti-cancer medicines. (C) 2012 Elsevier Inc. All rights reserved.