Estrogen response system in the reproductive tract of the male turtle: An immunocytochemical study

Estrogen response system in the reproductive tract of the male turtle: An immunocytochemical study
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DOI:
10.1016/j.ygcen.2006.06.012
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发表时间:
2007-03-01
影响因子:
2.7
通讯作者:
Hess, Rex A.
Hess, Rex A.
中科院分区:
医学3区
文献类型:
--
作者:
Gist, Daniel H.;Bradshaw, Suzanne;Hess, Rex A.

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用免疫细胞化学方法检测了雄性龟(Trachemys scripta)的部分生殖道,以寻找产生雌激素激素并对其产生反应的证据(通过P450芳香化酶和雌激素受体的表达)。芳香酶在睾丸的支持细胞和间质细胞中均有表达,在生精周期中表达水平不同,在静止睾丸中表达最高,在生殖细胞减数分裂时表达最低。内质网α存在于精小管周围的间质细胞以及出管(睾丸网、传出小管和附睾)的上皮细胞中。在睾丸的精原细胞和支持细胞以及外管上皮细胞中均发现ER - β免疫反应性。(c) 2006爱思唯尔公司版权所有。
Portions of the reproductive tract of the male (Trachemys scripta) turtle were examined by immunocytochemistry for evidence of the capacity to produce and respond to estrogen hormones (via the expression of P450 aromatase and estrogen receptors). Aromatase was detected in both the Sertoli and Leydig cells of the testis and was expressed at different levels during the spemiatogenic cycle, being highest in the quiescent testis and lowest during germ cell meiosis. ER alpha was found in the Leydig cells surrounding the seminiferous tubules as well as in the epithelial cells of the excurrent canals (rete testis, efferent ductule, and epididymis). ER beta immunoreactivity was found in both the spermatogonia and Sertoli cells in the testis, and in the epithelial cells of excurrent canals. (c) 2006 Elsevier Inc. All rights reserved.