Evidence for control of nitrogen metabolism by a START-dependent mechanism in Saccharomyces cerevisiae.
Evidence for control of nitrogen metabolism by a START-dependent mechanism in Saccharomyces cerevisiae.
复制标题
酿酒酵母中 START 依赖性机制控制氮代谢的证据。
DOI：
10.1007/s00438-003-0957-5
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Polymenis,M
中科院分区：
文献类型：
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作者：
Bryan,BA;McGrew,E;Lu,Y;Polymenis,M
一般认为细胞的生长和代谢调节细胞的分裂，反之则不然。在这里，我们研究了在恒化器中连续培养条件下生长的酵母细胞。我们发现，G1期细胞周期蛋白的损失，或细胞周期蛋白依赖性激酶Cdc28p的失活，降低了谷氨酸合酶（Glt1p），氮同化的关键酶的活性。我们还提出的证据表明，G1细胞周期蛋白依赖的控制Glt1p可能涉及Jem1p，DnaJ型伴侣。我们的研究结果表明，START的完成可能与氮代谢有关。
It is generally thought that cell growth and metabolism regulate cell division and notvice versa. Here, we examinedSaccharomyces cerevisiaecells growing under conditions of continuous culture in a chemostat. We found that loss of G1 cyclins, or inactivation of the cyclin-dependent kinase Cdc28p, reduced the activity of glutamate synthase (Glt1p), a key enzyme in nitrogen assimilation. We also present evidence indicating that the G1 cyclin-dependent control of Glt1p may involve Jem1p, a DnaJ-type chaperone. Our results suggest that completion of START may be linked to nitrogen metabolism.