MITOCHONDRIAL-DNA VARIABILITY IN DROSOPHILA-SIMULANS - QUASI ABSENCE OF POLYMORPHISM WITHIN EACH OF THE 3 CYTOPLASMIC RACES
MITOCHONDRIAL-DNA VARIABILITY IN DROSOPHILA-SIMULANS - QUASI ABSENCE OF POLYMORPHISM WITHIN EACH OF THE 3 CYTOPLASMIC RACES
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DOI：
10.1038/hdy.1988.133
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发表时间：
1988-12-01
期刊：
影响因子：
3.8
通讯作者：
DAVID, JR
中科院分区：
文献类型：
--
作者：
BABAAISSA, F;SOLIGNAC, M;DAVID, JR
本文对采自中国南方稻区的144个同雌株系的线粒体DNA进行了核苷酸变异分析。用10-15种限制性内切酶分析了simulans，用一种或几种酶研究了73个品系。所有克隆均分布在3个线粒体基因组中，siI、siII和siIII。这些类型是异地的，可以定义地理种族。混合种群只出现在马达加斯加和留尼汪岛，在那里siII和siIII被发现在一起。在用10种酶检测到的40个位点中，编码区的变异性极低，根据类型有一个或没有多态性限制性位点。对照富含A + T的区域在长度和限制性位点上变化更大，并且允许指定亚型。几个品系在基因组长度上是异质的。这些结果是相关的物种的进化历史，其最近在全球范围内的扩展和可能的创始人在每一个三种类型的起源的影响。
Nucleotide variability of mtDNA extracted from 144 isofemale lines collected in the whole range of D. simulans was analysed with 10-15 restriction enzymes and 73 lines were studied using one or a few enzymes. All clones were distributed into 3 mitochondrial genomes, siI, siII and siIII. These types are allopatric and can define geographic races. Mixed populations occur only in Madagascar and Reunion, where siII and siIII are found together. Among 40 sites detected with 10 enzymes, the variability of the coding region is extremely low, with one or no polymorphic restriction sites depending on the type. The control A + T-rich region is more variable in length and in restriction sites, and allows subtypes to be designated. Several lines were heteroplasmic for the length of the genome. These results are relevant to the evolutionary history of the species, its recent worldwide extension and to probable founder effects at the origin of each of the three types.