Porphyromonas gingivalis Ferrous Iron Transporter FeoB1 Influences Sensitivity to Oxidative Stress
Porphyromonas gingivalis Ferrous Iron Transporter FeoB1 Influences Sensitivity to Oxidative Stress
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DOI：
10.1128/iai.00108-09
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发表时间：
2010-02-01
影响因子：
3.1
通讯作者：
Lewis, Janina P.
中科院分区：
文献类型：
--
作者：
Anaya-Bergman, Cecilia;He, Jia;Lewis, Janina P.
牙龈卟啉单胞菌FeoB1是一种亚铁转运蛋白。对牙周病原体的亲本菌株和feoB1缺陷菌株的分析表明，feoB1缺陷突变菌株具有更强的氧化应激生存能力。具体地说，与亲本菌株相比，暴露在过氧化氢中的突变菌株的存活率提高了33%，暴露在大气中的氧气中的存活率提高了5%。有趣的是，在FeoB1缺陷突变体的情况下，细胞内存活的能力也增加了五倍。我们的数据表明，虽然FeoB1蛋白是牙龈假单胞菌获得亚铁所必需的，但它也对细菌在氧化应激条件下的生存有不利影响。最后，我们证明了feoB1的表达不依赖于铁，在宿主细胞存在的情况下显著降低，这与它在细菌生存中所起的有害作用是一致的。
Porphyromonas gingivalis FeoB1 is a ferrous iron transporter. Analysis of parental and feoB1-deficient strains of the periodontal pathogen revealed that the feoB1-deficient mutant strain had an increased ability to survive oxidative stress. Specifically, survival of the mutant strain was increased 33% with exposure to peroxide and 5% with exposure to atmospheric oxygen compared to the parental strain. Interestingly, the ability to survive intracellularly also increased fivefold in the case of the feoB1-deficient mutant. Our data suggest that although the FeoB1 protein is required for ferrous iron acquisition in P. gingivalis, it also has an adverse effect on survival of the bacterium under oxidative stress conditions. Finally, we show that feoB1 expression is not iron dependent and is dramatically reduced in the presence of host cells, consistent with the observed deleterious role it plays in bacterial survival.