In vivo imaging of radiation-induced apoptosis in follicular lymphoma patients
In vivo imaging of radiation-induced apoptosis in follicular lymphoma patients
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DOI：
10.1016/j.ijrobp.2003.11.017
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发表时间：
2004-07-01
影响因子：
7
通讯作者：
Verheij, M
中科院分区：
文献类型：
--
作者：
Haas, RLM;de Jong, D;Verheij, M
目的：评价99（m）Tc-膜联蛋白-V（TAV）显像在监测滤泡性淋巴瘤（FL）patients.Patients和方法：11例FL患者（7名女性和4名男性;中位年龄，58岁;范围，42-80岁）与复发性疾病进行TAV成像之前和24小时后的最后一部分的2 X 2戈伊涉及领域放疗方案。连续5天进行细针穿刺细胞学检查，以确定细胞凋亡检测的最佳时间窗，并确认应答的细胞凋亡性质。放射性药物给药后4小时进行TAV造影（全身研究和辐照部位SPECT）。以半定量方式将肿瘤摄取评分为不存在（-）弱（+/-）、存在（+）或强（+ +），并对细胞学切片进行相应分类。反应评估进行后1周和4周无论是在完全性和速度remedition.Results：基线TAV摄取不存在6例和弱5例。序贯细胞学检查表明，凋亡评估的最佳时间段是2 × 2戈伊方案最后一次分割后24 - 48小时。所有患者的基线细胞学检查与基线TAV一致。低剂量照射后出现的凋亡特征（核染色质凝聚、边集和凋亡小体形成）与所有患者的照射反应相匹配。除1例患者外，所有患者的治疗后TAV摄取均与治疗后细胞学检查相匹配。结论：2 × 2戈伊介入野放疗后，肿瘤对99（m）Tc-Annexin-V的摄取增加。这种增加与细胞学确定的凋亡形态学的出现一致，并且与临床结果相关。在该方案的第4天可以观察到凋亡细胞死亡，如果是这样，则预测在1周内完全缓解。(C)2004年爱思唯尔公司
Purpose: To evaluate 99(m)Tc-Annexin-V (TAV) scintigraphy in monitoring radiation-induced apoptotic cell death in follicular lymphoma (FL) patients.Patients and Methods: Eleven FL patients (7 female and 4 male; median age, 58 years; range, 42-80 years) with recurrent disease underwent TAV imaging before and 24 hours after the last fraction of the 2 X 2 Gy involved field radiotherapy regimen. Fine-needle aspiration cytology was performed on 5 consecutive days to determine the optimal time window for apoptosis detection and to confirm the apoptotic nature of the response. The TAV scintigraphy (total body studies and SPECT of the irradiated sites) was performed 4 hours after the administration of the radiopharmaceutical. Tumor uptake was scored in a semiquantitative manner as absent (-) weak (+/-), present (+), or intense (+ +) with corresponding categories for the cytologic slides. Response evaluation was performed after 1 week and 4 weeks both in terms of completeness and speed of remission.Results: Baseline TAV uptake was absent in 6 and weak in 5 patients. Sequential cytology indicated that the optimal time period for apoptosis assessment was between 24 and 48 hours after the last fraction of the 2 X 2 Gy regimen. Baseline cytology was concordant with baseline TAV in all patients. Apoptotic feature appearance (nuclear chromatin condensation, margination and apoptotic body formation) after low-dose irradiation matched the irradiation response in all patients. In all but 1 patient the posttreatment TAV uptake matched the posttreatment cytology. In these 10 patients the cytology and TAV results correlated with the type and onset of the clinical response.Conclusion: Tumor 99(m)Tc-Annexin-V uptake can be increased after 2 X 2 Gy involved field radiotherapy. This increase was concordant with the appearance of apoptotic morphology as determined by cytology, and correlated with the clinical outcome. Apoptotic cell death can be observed on Day 4 of this regimen and if so predicts a complete remission within 1 week. (C) 2004 Elsevier Inc.