An in vivo murine model of continuous intramedullary infusion of polyethylene particles
An in vivo murine model of continuous intramedullary infusion of polyethylene particles
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DOI：
10.1016/j.biomaterials.2008.05.031
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发表时间：
2008-09-01
期刊：
影响因子：
14
通讯作者：
Goodman, Stuart B.
中科院分区：
文献类型：
--
作者：
Ma, Ting;Huang, Zhinong;Goodman, Stuart B.
磨损碎屑影响全关节置换术（TJR）的初始骨整合和随后的骨重建。为了研究与连续产生的颗粒相关的复杂级联，一个强大的动物模型是必不可少的。迄今为止，还没有将连续输送的颗粒结合到髓内矫形植入物的动物模型。在这项研究中，我们使用皮下渗透泵成功地将之前从关节模拟器测试中分离的临床相关的超高分子量聚乙烯颗粒注入小鼠股骨的髓内空间4周。通过microCT检测到UHMWPE输注4周后的骨体积减少。UHMWPE颗粒还改变了灌注股骨中碱性磷酸酶的表达水平。向鼠骨-植入物界面连续输注颗粒模拟了人体局部聚合物磨损颗粒生成和输送的临床情况，可用于进一步研究与磨损碎屑颗粒相关的生物学过程。(C)2008爱思唯尔有限公司保留所有权利。
Wear debris affects both initial osseointegration and subsequent bone remodeling of total joint replacements (TJRs). To study the complex cascade associated with the continuous generation of particles, a robust animal model is essential. To date, an animal model that incorporates continuously delivered particles to an intramedullary orthopaedic implant has not been available. In this study, we successfully infused clinically relevant ultra high molecular weight polyethylene particles, previously isolated from joint simulator tests, to the intramedullary space of the mouse femur for 4 weeks using a subcutaneous osmotic pump. Reduction of bone volume following the 4-week infusion of UHMWPE was detected by microCT. UHMWPE Particles also changed the level of Alkaline Phosphatase expression in the infused femurs. Continuous infusion of particles to the murine bone-implant interface simulated the clinical scenario of local polymer wear particle generation and delivery in humans and can be used to further study the biological processes associated with wear debris particles. (C) 2008 Elsevier Ltd. All rights reserved.