Human bone marrow colony growth in agar‐gel
Human bone marrow colony growth in agar‐gel
复制标题
人骨髓集落在琼脂凝胶中的生长
DOI：
10.1002/jcp.1040760111
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发表时间：
1970
影响因子：
5.6
通讯作者：
W. Robinson
中科院分区：
文献类型：
--
作者：
B. L. Pike;W. Robinson
描述了在琼脂-凝胶培养基中生长人骨髓细胞集落的技术。使用含有1 × 106个正常人外周白色血细胞的“饲养层”作为集落生长的刺激物。将人骨髓穿刺液收集在肝素化注射器中，并以2 × 105个细胞接种在“饲养层”上。正常人骨髓每2 × 105个细胞接种产生32-102个集落。菌落几乎完全是粒细胞性的。集落的生长速率比小鼠骨髓慢，但集落在第12-16天达到相当的大小（500-1500个细胞）。
A technique for growing human bone marrow cell colonies in agar‐gel medium is described. “Feeder layers” containing 1 × 106 normal human peripheral white blood cells are used as the stimulus for colony growth. Human bone marrow aspirates are collected in heparinized syringes and plated as 2 × 105 cells on “feeder layers.” Normal human bone marrow yields 32–102 colonies per 2 × 105 cells plated. Colonies are almost exclusively granulocytic. Growth rate of colonies is slower than with mouse bone marrow but colonies reach a comparable size (500–1500 cells) at days 12–16.