The effects of co-administration of butter on the absorption, metabolism and excretion of catechins in rats after oral administration of tea polyphenols.
The effects of co-administration of butter on the absorption, metabolism and excretion of catechins in rats after oral administration of tea polyphenols.
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DOI：
10.1039/c5fo00114e
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发表时间：
2015-07
期刊：
影响因子：
6.1
通讯作者：
Liang Zhang;Yuhui Han;Liwei Xu;Yuhong Liang;Xin Chen;Junsong Li;Xiaochun Wan
中科院分区：
文献类型：
--
作者：
Liang Zhang;Yuhui Han;Liwei Xu;Yuhong Liang;Xin Chen;Junsong Li;Xiaochun Wan
在中国西南地区，茶多酚通常以酥油茶的方式利用。茶多酚抑制体内脂肪酸的吸收和生物合成，但黄油对茶多酚药代动力学的影响较少引起关注。采用超高效液相色谱-串联质谱法（UHPLC-MS/MS）对大鼠口服茶多酚及其与黄油配伍后血浆、粪便和胆汁中的儿茶素进行了定量分析。与单一茶多酚处理相比，黄油共同给药后游离EGCG、EGC、EC、GCG、GC和ECG的最大血浆浓度（Cmax）显著降低。游离EGCG、EGC、EC、GC和ECG的平均停留时间（MRT）也显著延长。当用β-葡萄糖醛酸酶和芳基硫酸酯酶处理血浆样品时，总儿茶素（游离和结合形式）的药代动力学参数不受黄油共同给药的影响。结果表明，黄油对儿茶素的吸收总量没有影响，但改变了儿茶素的代谢。此外，粪便儿茶素显着增加黄油。粪总排泄量和排泄率均显著增加。与黄油同时给药可显著增加胆汁中EGCG、EGC、EC、GCG和GC的排泄。综上所述，黄油通过降低游离儿茶素的血浆浓度而增加结合儿茶素的血浆浓度来改变儿茶素在体内的代谢。
In Southwest China, tea polyphenols are usually utilized by way of butter tea. Tea polyphenols inhibit the absorption and biosynthesis of fatty acids in vivo, but the effects of butter on the pharmacokinetics of tea polyphenols have drawn less concern. A rapid UHPLC-MS/MS method was used to quantitatively determine the catechins in the plasma, feces and bile of rats after the oral administration of tea polyphenol or its combination with butter. In comparison with the single tea polyphenol treatment, the maximum plasma concentrations (Cmax) of the free EGCG, EGC, EC, GCG, GC and ECG significantly decreased after the co-administration of butter. The mean residence times (MRT) of the free EGCG, EGC, EC, GC and ECG were also significantly prolonged. When the plasma samples were treated with β-glucuronidase and arylsulfatase, the pharmacokinetic parameters of the total catechins (free and conjugated forms) were not affected by the co-administration of butter. These results indicated that the total absorption of catechins was not affected by butter, but the metabolism of catechins had been changed. Furthermore, the fecal catechins were significantly increased by butter. The total fecal amount and excretion ratio of all catechins were increased highly. The biliary excretion of EGCG, EGC, EC, GCG and GC was significantly increased by the co-administration of butter. To sum up, the butter changed the metabolism of catechins in vivo by decreasing the plasma concentration of the free catechins but increasing the conjugated catechins.