Blood group antigen recognition by a solute-binding protein from a serotype 3 strain of Streptococcus pneumoniae.
Blood group antigen recognition by a solute-binding protein from a serotype 3 strain of Streptococcus pneumoniae.
复制标题
DOI：
10.1016/j.jmb.2009.03.012
复制
发表时间：
2009-05-01
影响因子：
5.6
通讯作者：
Boraston, Alisdair B.
中科院分区：
文献类型：
--
作者：
Higgins, Melanie A.;Abbott, D. Wade;Boulanger, Martin J.;Boraston, Alisdair B.
关键词：
肺炎链球菌是一种常见的细菌病原体，以其引起急性呼吸道疾病（肺炎）、耳部感染和其他严重疾病的能力而闻名。这种革兰氏阳性细菌依靠其碳水化合物代谢能力在其宿主中发挥全部毒力；然而，聚糖可以攻击的靶标范围目前还不完全清楚。已知肺炎链球菌具有一个病灶利用操纵子，该操纵子在TIGR4菌株中起毒力作用。在这里，我们确定了第二种类型的病灶利用操纵子，它存在于肺炎链球菌菌株的一个亚群中，包括血清3型菌株SP3-BS71。该操纵子包含一个转运体，转运体带有一个溶质结合蛋白FcsSBP（聚焦溶质结合蛋白），它与可溶性a -和b -抗原三糖紧密相互作用（Ka ~1 × 106 M−1），并特异性地与这两种糖相互作用，但在这两种糖之间没有选择性。fcsbp与a -三糖抗原复合物的结构（确定为2.35 Å）揭示了其与该糖的还原端结合的模式，从而突出了该蛋白对可溶性血型抗原配体的需求。总的来说，本报告揭示了某些肺炎链球菌菌株迄今未知的运输和潜在代谢宿主组织血型抗原碳水化合物的能力。
Streptococcus pneumoniae is a common bacterial pathogen that is well-known for its ability to cause acute respiratory disease (pneumonia), ear infections, and other serious illnesses. This Gram-positive bacterium relies on its carbohydrate metabolizing capabilities for full virulence in its host; however, the range of glycan targets that it can attack is presently not fully appreciated. S. pneumoniae is known to have a fucose utilization operon that in the TIGR4 strain plays a role in its virulence. Here we identify a second type of fucose utilization operon that is present in a subset of S. pneumoniae strains, including the serotype 3 strain SP3-BS71. This operon contains a transporter with a solute-binding protein, FcsSBP (Fucose Solute Binding Protein), that interacts tightly (Ka ~1 × 106 M−1) and specifically with soluble A- and B-antigen trisaccharides but displays no selectivity between these two sugars. The structure of the FcsSBP in complex with the A-trisaccharide antigen, determined to 2.35 Å, reveals its mode of binding to the reducing end of this sugar thus highlighting this protein’s requirement for soluble blood-group antigen ligands. Overall, this report exposes a heretofore unknown capability of certain S. pneumoniae strains to transport and potentially metabolize the histo-blood group antigen carbohydrates of its host.
登录
查看更多内容
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1107/s0907444902005486
发表时间：
2002-06-01
影响因子：
2.2
作者：
Evans, G;Bricogne, G
通讯作者：
Bricogne, G
影响因子：
4.2
作者：
Embry, Addie;Hinojosa, Ernesto;Orihuela, Carlos J.
通讯作者：
Orihuela, Carlos J.
影响因子：
4.8
作者：
Ficko-Blean, Elizabeth;Boraston, Alisdair B.
通讯作者：
Boraston, Alisdair B.
影响因子：
4.1
作者：
Boraston, AB;Ghaffari, M;Kilburn, DG
通讯作者：
Kilburn, DG