An Automated and High-Throughput Immunoaffinity Magnetic Bead-Based Sample Clean-Up Platform for the Determination of Aflatoxins in Grains and Oils Using UPLC-FLD
An Automated and High-Throughput Immunoaffinity Magnetic Bead-Based Sample Clean-Up Platform for the Determination of Aflatoxins in Grains and Oils Using UPLC-FLD
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DOI：
10.3390/toxins11100583
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发表时间：
2019-10-01
期刊：
影响因子：
4.2
通讯作者：
Wang, Songxue
中科院分区：
文献类型：
--
作者：
Xuan, Zhihong;Ye, Jin;Wang, Songxue
样品清理仍然是整个黄曲霉毒素分析过程中最耗时和最容易出错的步骤。在此，我们开发了一个自动化的高通量样品清理平台，该平台使用的是一次性的、具有成本效益的免疫亲和磁珠基试剂盒。在优化条件下，自动化方法无需任何离心或过滤步骤，只需不到30分钟即可同时纯化20个样品。当与荧光检测的超高效液相色谱相结合时，该分析方法具有良好的准确度和精密度，并且具有出色的效率。此外，在六个实验室进行了一项实验室间研究，以验证新方案。平均回收率、重复性、再现性和Horwitz比值分别在91.9% ~ 107.4%、2.5% ~ 7.4%、2.7% ~ 10.6%和0.26% ~ 0.90之间。结果表明，开发的样品清理平台是最广泛采用的谷物和油脂中AFTs清理程序的可靠替代方案。
Sample clean-up remains the most time-consuming and error-prone step in the whole analytical procedure for aflatoxins (AFTs) analysis. Herein, an automated and high-throughput sample clean-up platform was developed with a disposable, cost-effective immunoaffinity magnetic bead-based kit. Under optimized conditions, the automated method takes less than 30 min to simultaneously purify 20 samples without requiring any centrifugation or filtering steps. When coupled to ultra-high performance liquid chromatography with fluorescence detection, this new analysis method displays excellent accuracy and precision as well as outstanding efficiency. Furthermore, an interlaboratory study was performed in six laboratories to validate the novel protocol. Mean recovery, repeatability, reproducibility, and Horwitz ratio values were within 91.9%-107.4%, 2.5%-7.4%, 2.7%-10.6%, and 0.26%-0.90, respectively. Results demonstrate that the developed sample clean-up platform is a reliable alternative to most widely adopted clean-up procedures for AFTs in cereals and oils.