Synthesis of prostaglandins by cultured rat hearts myocytes and cardiac mesenchymal cells.
Synthesis of prostaglandins by cultured rat hearts myocytes and cardiac mesenchymal cells.
复制标题
培养的大鼠心肌细胞和心脏间充质细胞合成前列腺素。
DOI：
10.1016/0022-2828(80)90099-1
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发表时间：
1980
影响因子：
5
通讯作者：
Needleman,P
中科院分区：
文献类型：
--
作者：
Ahumada,GG;Sobel,BE;Needleman,P
Ischemia, trauma and hormonal stimulation elicit the release of prostaglandins (PGs) from the heart. Although PGI2is synthesized by coronary arteries, the capacities for PG synthesis of individual types of cells comprising the heart have not been elucidated. Accordingly, synthesis of prostaglandins by cultured rat cardiac myocytes and mesenchymal cells was evaluated by radiochromatography of products obtained by incubating cells with [1-14C]arachidonate, and verified by assessing the effects of cell incubation medium on platelet aggregation. Cultured mesenchymal cells synthesized PGs E2, F2αand 6-keto-F1α, a metabolite of PGI2(2076 ± 183, 1284 ± 158 and 1194 ± 152 dpm/mg protein/30 min, respectively). Medium from mesenchymal cells inhibited platelet aggregation, an effect abolished by preincubating the cells with indomethacin, further indicating that these cells synthesized PGI2. Cardiac myocytes synthesized PGE2and PGF2α(952 ± 227 and 287 ± 104 dpm/mg protein/30 mins, respectively), but no PGI2. Medium from myocytes did not inhibit platelet aggregation. Prostaglandins D2, A2and thromboxane were not synthesized by either type of cell. Thus, PGI2is synthesized by cardiac mesenchymal cells and the hitherto uncharacterized sources of PGE2and PGF2αfound in coronary sinus effluent may include cardiac myocytes as well as mesenchymal cells.
