Improving lysine production through construction of anEscherichia colienzyme-constrained model
Improving lysine production through construction of anEscherichia colienzyme-constrained model
复制标题
通过构建大肠杆菌酶约束模型提高赖氨酸产量
DOI：
10.1002/bit.27485
复制
发表时间：
2020
影响因子：
3.8
通讯作者：
Chen Xiulai
中科院分区：
文献类型：
--
作者：
Ye Chao;Luo Qiuling;Guo Liang;Gao Cong;Xu Nan;Zhang Li;Liu Liming;Chen Xiulai
Microbial cell factories are widely used for the production of high‐value chemicals. However, maximizing production titers is made difficult by the complicated regulatory mechanisms of these cell platforms. Here,kcatvalues were incorporated to construct anEscherichia colienzyme‐constrained model. The resulting ec_iML1515 model showed that the protein demand and protein synthesis rate were the key factors affecting lysine production. By optimizing the expression of the 20 top‐demanded proteins, lysine titers reached 95.7 ± 0.7 g/L, with a 0.45 g/g glucose yield. Moreover, adjusting NH4+and dissolved oxygen levels to regulate the synthesis rate of energy metabolism‐related proteins caused lysine titers and glucose yields to increase to 193.6 ± 1.8 g/L and 0.74 g/g, respectively. The ec_iML1515 model provides insight into how enzymes required for the biosynthesis of certain products are distributed between and within metabolic pathways. This information can be used to accurately predict and rationally design lysine production.
