Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential
Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential
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DOI：
10.1016/j.jep.2008.10.028
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发表时间：
2009-01-21
影响因子：
5.4
通讯作者：
Patwardhan, Bhushan
中科院分区：
文献类型：
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作者：
Gautam, Manish;Saha, Santanu;Patwardhan, Bhushan
民族药理学相关性：芦笋根总状花序Willd（Shatavari白话）被广泛用于阿育吠陀作为Rasayana用于免疫刺激，催乳剂也用于治疗溃疡和癌症等疾病。各种研究表明Shatavari根提取物和制剂的免疫调节特性。研究目的：研究标准化总状芦笋根水提取物（ARE）对SRBC致敏动物全身Th 1/Th 2免疫的影响。材料和方法：我们使用HPTLC定量甾体皂苷（Shatavarin IV，Immunoside（R））和流式细胞术研究ARE对Th 1/Th 2免疫的影响。SRBC特异性抗体滴度和DTH反应也分别作为Th 2和Th 1反应的标志物进行监测。我们还研究了淋巴细胞增殖。结果：用ARE（100 mg/（kgb.w.p.o.））结果表明，该制剂能显著提高T细胞活化率，并能显著提高CD_3（+）和CD_4/CD_8（+）比值。ARE处理的动物显示出Th 1（IL-2，IFN-g）和Th 2（IL-4）细胞因子的显著上调，表明其混合的Th 1/Th 2佐剂活性。与此一致，ARE也显示出更高的抗体滴度和DTH反应。ARE与LPS、ConA或SRBC联合应用，对活化的淋巴细胞有明显的增殖作用。结论：ARE的Th 1/Th 2混合活性支持其免疫佐剂作用。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Roots of Asparagus racemosus Willd (Shatavari in vernacular) are widely used in Ayurveda as Rasayana for immunostimulation, galactogogue as also in treatment of conditions like ulcers and cancer. Various studies have indicated immunomodulatory properties of Shatavari root extracts and formulations.Aim of the study: To study the effect of standardized Asparagus racemosus root aqueous extract (ARE) on systemic Th1/Th2 immunity of SRBC sensitized animals.Materials and methods: We used HPTLC to quantify steroidal saponins (Shatavarin IV, Immunoside (R)) and flow cytometry to study effects of ARE on Th1/Th2 immunity. SRBC specific antibody titres and DTH responses were also monitored as markers of Th2 and Th1 responses, respectively. We also studied lymphocyte proliferation. Cyclosporin, cyclophosphamide and levamisole were used as controls.Results: Treatment with ARE (100mg/(kgb.w.p.o.)) resulted in significant increase of CD3(+) and CD4/CD8(+) percentages suggesting its effect on T cell activation. ARE treated animals showed significant up-regulation of Th1 (IL-2, IFN-g) and Th2 (IL-4) cytokines suggesting its mixed Th1/Th2 adjuvant activity. Consistent to this, ARE also showed higher antibody titres and DTH responses. ARE, in combination with LPS, Con A or SRBC, produced a significant proliferation suggesting effect on activated lymphocytes.Conclusion: The study suggests mixed Th1/Th2 activity of ARE supports its immunoadjuvant potential. (C) 2008 Elsevier Ireland Ltd. All rights reserved.