Viola plant cyclotide vigno 5 induces mitochondria-mediated apoptosis via cytochrome C release and caspases activation in cervical cancer cells
Viola plant cyclotide vigno 5 induces mitochondria-mediated apoptosis via cytochrome C release and caspases activation in cervical cancer cells
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DOI：
10.1016/j.fitote.2015.12.021
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发表时间：
2016-03-01
期刊：
影响因子：
3.4
通讯作者：
Ghassempour, Alireza
中科院分区：
文献类型：
--
作者：
Esmaeili, Mohammad Ali;Abagheri-Mahabadi, Nazanin;Ghassempour, Alireza
环肽是一种独特的环肽家族，具有广泛的生物活性，包括子宫收缩、抗菌、抗癌和抗 HIV。最近从 Viola ignobilis 中报道了由 vigno 1-10 组成的 vigno cyclotides。在本研究中，我们研究了 vigno 5（一种来自 V. ignobilis 的天然环肽）对宫颈癌细胞的影响及其潜在机制。我们发现vigno 5处理的Hela细胞在24小时内以剂量依赖性方式被细胞凋亡杀死，并出现核皱缩、聚(ADP-核糖)聚合酶(PARP)裂解和DNA片段化。细胞凋亡的线粒体途径表明，细胞色素C从线粒体释放到细胞质中，与caspase-9和-3的激活以及聚（ADP-核糖）聚合酶（PARP）的裂解有关。总体而言，结果表明，vigno 5 部分通过线粒体途径诱导细胞凋亡，这与 Hela 细胞中细胞色素 C 的释放以及 caspase-9 和 -3 活性的升高有关。 (C) 2016 Elsevier B.V. 保留所有权利。
Cyclotides describe a unique cyclic peptide family that displays a broad range of biological activities including uterotonic, anti -bacteria, anti -cancer and anti -HIV. The vigno cyclotides consist of vigno 1-10 were reported recently from Viola ignobilis. In the present study, we examined the effects of vigno 5, a natural cyclopeptide from V. ignobilis, on cervical cancer cells and the underlying mechanisms. We found that vigno 5 -treated Hela cells were killed off by apoptosis in a dose -dependent manner within 24 h, and were characterized by the appearance of nuclear shrinkage, cleavage of poly (ADP -ribose) polymerase (PARP) and DNA fragmentation. The mitochondrial pathway of apoptosis revealed that cytochrome C is released from mitochondria to cytosol, associated with the activation of caspase-9 and -3, and the cleavage of poly (ADP -ribose) polymerase (PARP). Overall, the results indicate that vigno 5 induces apoptosis in part via the mitochondrial pathway, which is associated with a release of cytochrome C and elevated activity of caspase-9 and -3 in Hela cells. (C) 2016 Elsevier B.V. All rights reserved.