MECHANISM OF POLYMYXIN-B-ACTION AND SELECTIVITY TOWARD BIOLOGIC MEMBRANES
MECHANISM OF POLYMYXIN-B-ACTION AND SELECTIVITY TOWARD BIOLOGIC MEMBRANES
复制标题
DOI：
10.1021/bi00735a014
复制
发表时间：
1973-01-01
期刊：
影响因子：
2.9
通讯作者：
FEINGOLD, DS
中科院分区：
文献类型：
--
作者：
HSUCHEN, CC;FEINGOLD, DS
Chuen-Chin HsuChen 和 David S. Feingold* 摘要：使用水悬浮液（脂质体）中的脂质小球作为模型系统，研究了多粘菌素抗生素的作用选择性；捕获的葡萄糖标记物的释放是膜损伤的指标。据观察，多粘菌素 B 诱导的葡萄糖释放是一个区分具有各种“极头”结构的磷脂的特定过程。由磷脂酰乙醇胺制备的脂质体对多粘菌素B极其敏感，而由N-甲基取代的类似物(N-甲基磷脂酰乙醇胺、MY-二甲基磷脂酰乙醇胺、磷脂酰胆碱)制备的脂质体对抗生素不敏感。磷脂酰乙醇胺的 N-甲基化类似物保护含有磷脂酰乙醇胺的脂质体免受多粘菌素 B 的影响。这种保护的效率是类似物摩尔百分比的函数
Chuen-Chin HsuChen and David S. Feingold* abstract: The selectivity of action of the polymyxin antibiotics was studied using lipid spherules in aqueous suspension (liposomes) as a model system; the release of trapped glucose marker was the index of membrane damage. The polymyxin B induced glucose release was observed to be a specific process which differentiates among phospholipids with various “polar head” structures. Liposomes prepared from phos-phatidylethanolamine were extremely sensitive to polymyxin B while none of those prepared from the VV-methyl-substituted analogs (jV-methylphosphatidylethanolamine, MY-dimethylphosphatidylethanolamine, phosphatidylcholine) were sensi-tive to the antibiotic. The N-methylated analogs of phosphatidylethanolamine protected phosphatidylethanolamine-containing liposomes from polymyxin B. The efficiency of this protection was a function of the molar percent of the analog