Demonstration of altered fibroblast contractile activity in hypertensive heart disease
Demonstration of altered fibroblast contractile activity in hypertensive heart disease
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DOI：
10.1016/j.cardiores.2003.09.021
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发表时间：
2003-12-01
影响因子：
10.8
通讯作者：
Dembo, M
中科院分区：
文献类型：
--
作者：
Marganski, WA;De Biase, VM;Dembo, M
目的：本研究的目的是探讨成纤维细胞收缩活性改变与高血压性心脏病（HHD）的发病机制有关的观点。方法：使用牵引力显微镜技术对从正常血压 Wistar-Kyoto (WKY) 和自发性高血压 (SHR) 大鼠中分离的心脏成纤维细胞的细胞面积和收缩进行量化。结果：数据表明两种细胞类型之间存在显着的表型差异。例如，WKY 成纤维细胞施加的平均牵引应力约为 3.3 kPa，面积约为 2640 mum(2)。在相同条件下，SHR 成纤维细胞的面积约为 1.45 倍（p
Objective: The aim of this study is to investigate the idea that altered fibroblast contractile activity is involved in the pathogenesis of hypertensive heart disease (HHD). Methods: Cell area and contraction are quantified using the traction force microscopy technique for cardiac fibroblasts isolated from both normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Results: The data indicate that there are marked phenotypic differences between the two cell types. For instance, WKY fibroblasts exert an average traction stress of similar to 3.3 kPa and have an area of similar to 2640 mum(2). Under identical conditions the SHR fibroblasts have an area similar to 1.45 times larger (p