WHAT LIMITS RATE OF AN ENZYME-CATALYZED REACTION
WHAT LIMITS RATE OF AN ENZYME-CATALYZED REACTION
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DOI：
10.1021/ar50089a001
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发表时间：
1975-01-01
影响因子：
18.3
通讯作者：
CLELAND, WW
中科院分区：
文献类型：
--
作者：
CLELAND, WW
近年来，关于酶如何能够尽可能快地催化化学反应一直存在争议。然而，在这些争论中忽略了一个事实，即实际的化学反应很少限制酶促反应的速率，本说明的目的是表明蛋白质的构象变化和产物的释放（或者更可能是允许产物释放的构象变化）是常见的速率限制步骤。我们提出了几个有据可查的情况下，动力学的证据，使我们能够推导出的机制中的各个步骤的相对速率。最后，我们证明了热力学方程的酶催化反应速率的真实的上限
In recent years there has been some controversy about howenzymes manage to catalyze chemical reactions as fast as they do. Lost sight of in these arguments, however, has been the fact that the actual chemical reaction is seldom what limits the rate of an enzymatic reaction.It is the purpose of this Account to show that con-formation changes in the protein and release of products (or more likely, the conformation change that permits product release) are the common rate-limit-ing steps. We present several well-documented cases and the kinetic evidence that allows us to deduce the relative rates of the various steps in the mechanism. Finally we show that the real upper limit on the rate of an enzyme-catalyzed reaction of a thermodynamic