Second-harmonic generation microscopy for assessment of mesenchymal stern cell-seeded acellular dermal matrix in wound-healing
Second-harmonic generation microscopy for assessment of mesenchymal stern cell-seeded acellular dermal matrix in wound-healing
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DOI：
10.1016/j.biomaterials.2015.03.011
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发表时间：
2015-06-01
期刊：
影响因子：
14
通讯作者：
Zhan, Zhigang
中科院分区：
文献类型：
--
作者：
Wang, Qiannan;Jin, Ying;Zhan, Zhigang
直接皮内注射间充质干细胞（MSCs）和使用生物材料支架移植都是皮肤伤口修复的有前途的方法，但它们仍然不能产生完全类似于天然皮肤结构的皮肤。在这项研究中，我们结合这两种方法，使用脱细胞真皮基质（ADM）再细胞与MSC修复皮肤伤口的小鼠模型和双光子荧光（TPF）显微镜和二次谐波发生（SHG）显微镜，以评估这种疗法对伤口愈合的影响。骨髓间充质干细胞（BM-MSCs）经GFP标记后，通过与ADM共培养，接种于ADM（ADM-MSC）中。将ADM-MSC、ADM或盐水应用于小鼠切除皮肤伤口，并在治疗后第7、14、21天通过组织学检查和第1、3、5和21天通过TFP显微镜检查来评估伤口愈合。ADM-MSC促进愈合的作用显著高于单独使用ADM或生理盐水的治疗，因为它导致大量的新血管形成和完全的皮肤附件再生。此外，SHG显微成像技术被证明是一种有用的工具，用于监测愈合过程中伤口部位胶原蛋白网络的变化，并评估不同疗法的效果。(C)2015爱思唯尔有限公司版权所有。
Direct intra-skin injection of mesenchymal stem cells (MSCs) and the use of biomaterial scaffolds for grafts are both promising approaches of skin wound repair, however they still cannot generate skin that completely resembles the natural skin structures. In this study, we combined these two approaches by using acellular dermal matrix (ADM) recellularized with MSCs to repair cutaneous wounds in a murine model and two-photon fluorescence (TPF) microscopy and second-harmonic generation (SHG) microscopy to assess the effects of this therapy on wound healing. Bone marrow-derived mesenchymal stem cells (BM-MSCs) were tagged with GFP and seeded into ADM (ADM-MSC) via MSC and ADM co-culture. ADM-MSC, ADM or saline was applied to murine excisional skin wounds and wound-healing was evaluated by histological examination on days 7, 14, 21 and TFP microscopy on days 1, 3, 5 and 21 post-treatment. ADM-MSC promoted healing significantly more than treatment with ADM or saline alone, as it led to substantial neovascularization and complete skin appendage regeneration. Furthermore, the SHG microscopic imaging technique proved to be a useful tool for monitoring changes in the collagen network at the wound site during the healing process and assessing the effects of different therapies. (C) 2015 Elsevier Ltd. All rights reserved.