Humus bacteria of Norway spruce stands: plant growth promoting properties and birch, red fescue and alder colonizing capacity
Humus bacteria of Norway spruce stands: plant growth promoting properties and birch, red fescue and alder colonizing capacity
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DOI：
10.1111/j.1574-6941.2000.tb00679.x
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发表时间：
2000-02-01
影响因子：
4.2
通讯作者：
Haahtela, K
中科院分区：
文献类型：
--
作者：
Elo, S;Maunuksela, L;Haahtela, K
我们研究了挪威云杉林的腐殖质层为白桦（Betula pendula）、桤木（Alnus incana）和羊茅（Festuca rubra）提供有益根瘤菌的潜力，这是针叶林砍伐后先锋植被的代表。用pH为3.7 ~ 5.3的酸性云杉腐殖质接种无性系幼苗。长期施石灰处理地块腐殖质中的放线根繁殖体密度较高(10(3)g(-1))，而施氮处理地块的放线根繁殖体密度几乎为0（小于或等于10 g(-1)）。腐殖质中最常见的属有芽孢杆菌属、类芽孢杆菌属、节杆菌属、诺卡菌属、红球菌属和假单胞菌属。独立于预先施肥或施肥的。在幼苗根系中发现的分类群与腐殖质中的不同之处在于革兰氏阴性属假单胞菌、碱性单胞菌和共生单胞菌的流行。根在无氮培养基上富集培养产生芽孢杆菌和红球菌。固氮R. erythropolis和一种新的Paenibacillus，在16S rDNA全序列上与P. durus最接近，代表了根际固氮细菌的新类别。此外，在腐殖质中还发现了固氮菌。根区和腐殖质中含有高比例的拮抗植物致病性根丝胞菌。灰霉病菌和镰刀菌。拮抗菌株通常也产生铁载体和/或细胞壁降解酶，(C) 2000年欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
We studied the potential of the humus layer of the Norway spruce stands to supply beneficial rhizobacteria to birch (Betula pendula), alder (Alnus incana) and fescue grass (Festuca rubra), representatives of pioneer vegetation after clear-cutting of the coniferous forest. Axenically grown seedlings of these species were inoculated with the acid spruce humus, pH 3.7-5.3. Actinorhizal propagules, capable of nodulating alder, were present in high density (10(3) g(-1)) in humus of long-term limed plots, whereas plots with nitrogen fertilization contained almost none (less than or equal to 10 g(-1)). The genera most frequently found in the humus were Bacillus, Paenibacillus, Arthrobacter, Nocardia, Rhodococcus and Pseudomonas. independently of prior liming or fertilization of the plots. The taxa found in the seedling roots differed from that in humus by the prevalence of the Gram-negative genera Pseudomonas, Alcaligenes and Comamonas. Enrichment cultures of the roots on nitrogen-free media yielded Paenibacillus and Rhodococcus species. Nitrogen-fixing R. erythropolis and a novel Paenibacillus, closest by full sequence of 16S rDNA to P. durus, represented new classes of nitrogen-fixing rhizosphere bacteria. In addition, nitrogen-fixing R. fascians was found in the humus. The rhizoflora and humus contained high proportions of bacteria antagonistic towards plant pathogenic Rhizoctonia sp.. Botrytis cinerea and Fusarium culmorum. The antagonistic isolates also commonly produced siderophores and/or cell wall degrading enzymes, (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.