Effects of interleukin-1 and endotoxin in the forced swim and tail suspension tests in mice
Effects of interleukin-1 and endotoxin in the forced swim and tail suspension tests in mice
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DOI：
10.1016/j.pbb.2005.04.019
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发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Swiergiel, AH
中科院分区：
文献类型：
--
作者：
Dunn, AJ;Swiergiel, AH
雄性CD-1小鼠给予白细胞介素-1 β（IL-1 β）和细菌内毒素（脂多糖，LPS），随后进行测试。在悬尾试验（TST）、Porsolt强迫游泳试验（FST）和在开放场地中。将IL-1 β（100、300和1000 ng/小鼠）腹膜内（i. p.）试验前90分钟诱导TST中不动时间和FST中漂浮时间呈剂量依赖性增加。这些反应仅在较高剂量的IL-1 β（300和1000 ng）下具有统计学显著性。然而，所有三种剂量的IL-1 β均显著降低了旷场中的线交叉和后仰，并降低了食物摄入量和体重。LPS诱导的效应非常相似。1 μ g和5 μ g腹腔注射可增加TST中的不动时间和FST中的漂浮时间，但相同剂量可强烈抑制自发活动和体重。这些结果表明，IL-1 β和LPS均可诱导TST和FST中的抑郁样效应。然而，诱导这些变化所需的剂量减少了开放视野中的摄食和活动，因此在FST和TST中观察到的效应可归因于自发活动的普遍减少。因此，在这两个通常用于测试抗抑郁特性的动物试验中获得的结果并不能为IL-1抑郁假说提供有力的支持。(c)2005年爱思唯尔公司All rights reserved.
Male CD-1 mice were administered interleukin-1 beta (IL-1 beta) and bacterial endotoxin (lipopolysaccharide, LPS) and subsequently tested. in the tail suspension test (TST), the Porsolt forced swim test (FST), and in the open field. IL-1 beta (100, 300 and 1000 ng/mouse) injected intraperitoneally (i.p.) 90 min before the test induced a dose-dependent increase in the time spent immobile in the TST and the time spent floating in the FST. These responses were statistically significant only at the higher doses of IL-1 beta (300 and 1000 ng). Nevertheless, all three doses of IL-1 beta significantly decreased line crossings and rears in the open field and depressed food intake and body weight. Very similar effects were induced by LPS. Doses of 1 and 5 mu g i.p. increased immobility time in the TST and floating time in the FST, but the same doses strongly depressed locomotor activity and body weight.These results indicate that both IL-1 beta and LPS can induce depression-like effects in the TST and the FST. However, the doses necessary to induce these changes reduced feeding and activity in an open field, so that the effects observed in the FST and TST could be attributed to a general reduction in locomotor activity. Thus the results obtained in these two animal tests commonly used to test antidepressant properties do not provide strong support for an IL-1 hypothesis of depression. (c) 2005 Elsevier Inc. All rights reserved.