Occurrence, molecular and antimicrobial resistance of Enterococcus spp. isolated from raw cow's milk trade by street trading in Meknes city, Morocco
Occurrence, molecular and antimicrobial resistance of Enterococcus spp. isolated from raw cow's milk trade by street trading in Meknes city, Morocco
复制标题
DOI：
10.18683/germs.2018.1134
复制
发表时间：
2018-06-01
期刊：
影响因子：
2
通讯作者：
Moumni, Mohieddine
中科院分区：
文献类型：
--
作者：
Bouymajane, Aziz;Rhazi Filali, Fouzia;Moumni, Mohieddine
背景肠球菌属属于导致严重感染的一组病原体。本研究的目的是强调原料奶微生物污染，并提供数据的流行和耐药性的肠球菌属。方法在2015年5月至2016年4月期间，从梅克内市的街头商贩处收集了150头奶牛的生牛奶样本。对它们进行检查以鉴定肠球菌属。使用生化测试和16 S rRNA基因测序。结果150份标本中肠球菌阳性率为11.3%（17/150），其中粪肠球菌占64.7%，屎肠球菌占17.6%，硬肠球菌占11.8%，肠球菌占5.9%。药敏结果显示，所有肠球菌属均为耐药菌株。对氨苄青霉素有抗药性F. faecalis、粪肠球菌E. faecium、F. durans和F.对链霉素耐药率分别为52.9%（9/17）、11.8%（2/17）、11.8%（2/17）和5.9%（1/17）。所有分离的F. faecalis和F.粪菌对四环素有抗性。多重耐药指数在大多数肠球菌属中升高，结论本研究表明，人群食用的原料奶中存在肠球菌属菌株，该菌株对用于预防性养殖的抗生素具有耐药性。这就需要提高参与牛奶生产和销售的人的认识，以便采取措施，采用良好的卫生做法，合理使用畜牧业抗生素。
Background Enterococcus spp. belongs to a group of pathogens which are responsible for serious infections. This study aims at highlighting the raw milk microbiological contamination and at providing data for prevalence and antimicrobial resistance of Enterococcus spp. isolated from raw cow's milk marketed (without any pasteurization) by street traders.Methods During the period of May 2015 through April 2016, 150 cow's raw milk samples were collected from street traders in Meknes city. They were examined for the identification of Enterococcus spp. using biochemical tests and 16S rRNA gene sequencing. The antimicrobial susceptibility of the isolates was determined.Results The results showed that 11.3% (17/150) of samples were positive for the presence of Enterococcus spp., of which 64.7% were identified as Enterococcus faecalis, 17.6% as Enterococcus faecium, 11.8% as Enterococcus durans and 5.9% as Enterococcus hirae. The antimicrobial susceptibility showed that all Enterococcus spp. were resistant to ampicillin. The species F. faecalis, E. faecium, F. durans and F. hirae were resistant to streptomycin, with percentages of 52.9% (9/17), 11.8% (2/17), 11.8% (2/17), and 5.9% (1/17) respectively. All isolated strains of F. faecalis and F. faecium were resistant to tetracycline. The multiple antibiotic resistance index was elevated in the majority of Enterococcus spp., reaching values higher than 0.5, indicating a risk for public health.Conclusion This study shows that the raw milk consumed by the population is contaminated with strains of Enterococcus resistant to antibiotics used in breeding for prophylactic purposes. This requires raising the awareness of those involved in the production and marketing of milk, so as to take measures to apply good hygienic practices and rationalize the use of zootechnical antibiotics.