Differences between the fecal microbiota of coeliac infants and healthy controls.
Differences between the fecal microbiota of coeliac infants and healthy controls.
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发表时间：
2007-03
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通讯作者：
M. Collado;M. Calabuig;Y. Sanz
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作者：
M. Collado;M. Calabuig;Y. Sanz
乳糜泻（CD）是一种免疫介导的肠病，具有多因素病因，其特征是小肠粘膜的慢性炎症。尽管有证据表明肠道微生物群会导致其他慢性炎症性疾病，但其在克罗恩病中的可能作用尚未确定。在这项研究中，利用荧光原位杂交（FISH），通过培养依赖性和非培养方法研究了乳糜泻儿童和年龄匹配对照的粪便微生物群的组成。通过培养方法进行分析时，乳糜泻患者粪便样本中的拟杆菌、梭菌和葡萄球菌水平显着高于健康受试者（p < 0.05）。拟杆菌-普雷沃氏菌、溶组织梭菌、直肠真杆菌-C 的数量。通过 FISH 分析时，乳糜泻婴儿粪便样本中的球状菌、Atopobium 和硫酸盐还原菌群也显着较高 (p < 0.05)。通过两种类型的分析，健康对照中的双歧杆菌计数往往较高，但差异并不显着。这是第一份关于确定导致活动性 CD 儿童肠道微生态改变的特定细菌群的报告。在乳糜泻患者中检测到的细菌模式与 CD 相关的流行病学数据和代谢偏差相关，并且涉及与其他慢性炎症性疾病相关的细菌群。
Coeliac disease (CD) is an immune-mediated enteropathy with a multifactorial aetiology, characterized by chronic inflammation of the small intestinal mucosa. Although evidence suggests that the gut microbiota contributes to other chronic inflammatory disorders, its possible role in CD has not been determined. In this study, the composition of the fecal microbiota of coeliac children and age-matched controls was investigated by culture-dependent and -independent methodologies, using fluorescent in situ hybridization (FISH). The levels of Bacteroides, Clostridium and Staphylococcus were significantly higher (p < 0.05) in fecal samples from coeliac patients than in healthy subjects when analysed by culture methods. The numbers of Bacteroides-Prevotella, Clostridium histolyticum, Eubacterium rectale-C. coccoides, Atopobium, and sulfate reducing bacterial groups were also significantly higher (p < 0.05) in fecal samples from coeliac infants when analysed by FISH. The counts of Bifidobacterium tended to be higher in healthy controls by the two type of analysis but the differences were not significant. This is the first report on the identification of the specific bacterial groups responsible for alterations in the intestinal microecology of children with active CD. The bacterial pattern detected in coeliac patients, correlates with the epidemiological data and metabolic deviations associated with CD, and involve bacterial groups link to other chronic inflammatory disorders.