Ageing - Cloning of mice to six generations
Ageing - Cloning of mice to six generations
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DOI：
10.1038/35030301
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发表时间：
2000-09-21
期刊：
影响因子：
64.8
通讯作者：
Yanagimachi, R
中科院分区：
文献类型：
--
作者：
Wakayama, T;Shinkai, Y;Yanagimachi, R
小鼠已经通过核移植到去核卵母细胞中进行克隆，在这里，我们描述了小鼠在两个独立系中的四代和六代的重复克隆。连续的几代没有显示出过早衰老的迹象，这是通过粗略的行为参数来判断的，也没有证据表明染色体末端的端粒缩短了，这通常是细胞衰老的一个指标-事实上，这些端粒的长度似乎略有增加。这种增加是令人惊讶的，因为有丝分裂的数量大大超过了有性繁殖的动物，而且克隆的任何有害影响都可能在连续克隆的小鼠中被放大。我们的研究结果提供了一个新的方法来研究生物衰老。
Mice have been cloned by nuclear transfer into enucleated oocytes,,, and here we describe the reiterative cloning of mice to four and six generations in two independent lines. Successive generations showed no signs of prematureageing, as judged by gross behaviouralparameters, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence — in fact, these appeared to increase slightly in length. This increase is surprising, given that the number of mitotic divisions greatly exceeds that of sexually produced animals and that any deleterious effects of cloning might be expected to be amplified in sequentially cloned mice. Our results offer a new approach to the study of organismal ageing.