GS15, a 15-Kilodalton Golgi SolubleN-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (SNARE) Homologous to rbet1*
GS15, a 15-Kilodalton Golgi SolubleN-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (SNARE) Homologous to rbet1*
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DOI：
10.1074/jbc.272.32.20162
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发表时间：
1997-08
期刊：
影响因子：
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通讯作者：
Yue Xu;S. Wong;Zhang Tao;V. Subramaniam;W. Hong
中科院分区：
文献类型：
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作者：
Yue Xu;S. Wong;Zhang Tao;V. Subramaniam;W. Hong
Bet1p在酵母从内质网（ER）到高尔基体的囊泡运输中起重要作用，它是一种囊泡溶性乙基酰亚胺敏感因子蛋白受体（v-SNARE）。以前曾报道过一种与Bet1p相关的哺乳动物蛋白，称为rbet1。我们现在已经鉴定出一种新的哺乳动物蛋白，它与rbet1同源（28%氨基酸同源）。这种rbet1同源物的mRNA在大鼠组织中广泛表达。针对重组蛋白的亲和纯化多克隆抗体特异性识别高尔基膜中高度富集的15千道尔顿完整膜蛋白。间接免疫荧光显微镜显示该蛋白与不同细胞类型的高尔基体特异性相关。生化鉴定证实该蛋白的行为类似于一个SNARE，并被命名为GS15（高尔基SNARE，大小为15千道尔顿）。这些性质提高了GS15是一种新的SNARE介导与高尔基体相关的尚未定义的传输事件的可能性。
Bet1p plays an essential role in vesicular transport from the endoplasmic reticulum (ER) to the Golgi in yeast, and it functions as a vesicle solubleN-ethylmaleimide-sensitive factor protein receptor (v-SNARE). A mammalian protein related to Bet1p has been reported previously and was referred to as rbet1. We have now identified a new mammalian protein that is homologous to rbet1 (28% amino acid identity). mRNA for this rbet1 homologue is widely expressed in rat tissues. Affinity-purified polyclonal antibodies raised against recombinant protein specifically recognized a 15-kilodalton integral membrane protein highly enriched in Golgi membranes. Indirect immunofluorescence microscopy revealed that this protein is specifically associated with the Golgi apparatus in diverse cell types. Biochemical characterization established that this protein behaves like a SNARE and was named GS15 (Golgi SNARE with a size of 15 kilodaltons). These properties raise the possibility that GS15 is a novel SNARE mediating a yet to be defined transport event associated with the Golgi apparatus.