Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?
Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?
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DOI：
10.1042/bsr20150067
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发表时间：
2015-05-14
影响因子：
4
通讯作者：
Drewa T
中科院分区：
文献类型：
--
作者：
Bajek A;Gurtowska N;Gackowska L;Kubiszewska I;Bodnar M;Marszałek A;Januszewski R;Michalkiewicz J;Drewa T
统计分析显示，242例研究对象中58个标记物的抗原表达存在显著差异。吸脂方法对培养的ASCs（脂肪源性干细胞）的抗原谱无显著影响。脂肪源性干细胞（ASCs）具有高度分化和增殖潜力。然而，ASCs的表型表征仍然很困难。到目前为止，还没有对不同吸脂方法的ASCs标记物进行广泛的分析。因此，本研究的目的是分析242个表面标记物，并确定机械抽脂和超声辅助抽脂过程中获得的ASCs之间表型模式的差异。从健康供体中分离ASCs，通过机械和超声辅助吸脂，在标准培养基中培养至第二代。分化潜能和标志物表达被评估以确认细胞的间充质性质。然后使用BD LyoplateTM Human Cell Surface Marker Screening Panel。结果表明，两种ASCs群体均具有高表达ASCs特异性标志物的特征：cd9、CD10、CD34、CD44、CD49d、CD54、CD55、CD59、CD71，以及低表达CD11a、CD11c和CD144。此外，我们注意到在242个研究对象中，58个标记物的抗原表达存在显著差异。本研究首次发现吸脂方式的不同并不是影响人ASCs表面标志物表达的重要因素。
Statistical analysis revealed significant differences in antigen expression of 58 markers of the 242 studied. The method of liposuction has no significant impact on antigens profile in cultured ASCs (adipose-derived stem cells). Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.