Involvement of somatolactin in background adaptation of the cichlid fish Cichlasoma dimerus
Involvement of somatolactin in background adaptation of the cichlid fish Cichlasoma dimerus
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DOI：
10.1002/jez.a.273
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发表时间：
2006-05-01
影响因子：
2.8
通讯作者：
Vissio, PG
中科院分区：
文献类型：
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作者：
Cánepa, MM;Pandolfi, M;Vissio, PG
生长抑素(Somatolactin，SL)是一种仅存在于鱼类体内的脑下垂体激素，参与多种生理过程。SL在黑白背景(BB和WB)中暴露10天后，对二聚体(真骨纲，Perciformes)的作用进行了评估。同时分析α-黑素细胞刺激素(α-MSH)和黑色素浓缩激素(MCH)细胞的变化，并与SL进行比较。在暴露于BB的鱼类中观察到了黑色素的分散效应，而在暴露于WB的鱼类中观察到了黑色素的浓缩效应。免疫印迹显示有3条SL免疫反应(Ir)条带，分别为32、28和23.5kD。在32kD和28kD条带中，BB鱼的脑垂体SL-ir水平分别是WB鱼的2.66倍和2.67倍。由于其未知的身份，SL-Ir 23.5kD带不包括在分析中。此外，在BB状态下SL-ir细胞数目和面积显著增加(BB 22.73+/-1.46，WB 7.37+/-0.54和BB 27.39+/-1.00亩m(2)；WB：16.61+/-0.65亩m(2))。下丘脑MCH-ir神经元数目无明显差异。而核面积(BB 11.61+/-0.42 mm(2)，Wb 17.80+/-0.84 mm(2))差异有统计学意义。αMSH-ir细胞数量显著增加(BB 35.96+/-1.22，WB 24.36+/-1.04)，但细胞面积无明显差异。总之，这项研究提供了明确的证据，表明SL可能参与了硬骨鱼对背景颜色的适应，以及αCMSH和MCH。
Somatolactin (SL) is a pituitary hormone present exclusively in fish that is involved in different physiological processes. The role of SL was evaluated in Cichlasoma dimerus (Teleostei, Perciformes) exposed for 10 days to a black and white background (BB and WB). Changes in alpha-melanophore stimulating hormone (alpha MSH) and melanin concentrating hormone (MCH) cells were also analyzed for comparison with SL. A melanin dispersing effect was observed in fish exposed to a BB, while a concentrating one was observed in those exposed to a WB. By Western blot, three SL-immunoreactive (ir) bands (32, 28 and 23.5 kD) were evidenced. Pituitary SL-ir levels were 2.66- and 2.67-fold greater in the 32 Kd and 28 kD bands, respectively, in BB fish compared with those of WB fish. The SL-ir 23.5 Kd band was not included in the analysis because of its unknown identity. In addition, SL-ir cell number and area were significantly higher in the BB condition (BB 22.73 +/- 1.46, WB 7.37 +/- 0.54 and BB 27.39 +/- 1.00 mu m(2); WB: 16.61 +/- 0.65 mu m(2)). No significant differences were observed in the number of the hypothalamic MCH-ir neurons. However, a significant difference was observed in their nuclear area (BB 11.61 +/- 0.42 mu m(2), WB 17.80 +/- 0.84 mu m(2)). alpha MSH-ir cells showed a marked increased in number (BB 35.96 +/- 1.22, WB 24.36 +/- 1.04), but no significant differences were observed in the cell area. In conclusion, this study presented clear evidence towards a possible involvement of SL in the adaptation to background colors in teleost together with alpha CMSH and MCH.