Non-cellulosic mixed linkage beta-D-glucan in sorghum, Sorghum bicolor (L.) Moench--localization and biological activity studies.
Non-cellulosic mixed linkage beta-D-glucan in sorghum, Sorghum bicolor (L.) Moench--localization and biological activity studies.
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发表时间：
2000-02
影响因子：
0.6
通讯作者：
H. P. Ramesh;R. Tharanathan
中科院分区：
文献类型：
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作者：
H. P. Ramesh;R. Tharanathan
在高粱籽粒（Sorghum bicolor (L.) Moench）的染色切片中观察到 Calcoflor 和刚果红的荧光，特别是在亚糊粉层细胞中，表明存在混合连接 β-D-葡聚糖。在单层（约 20 微米厚）糊粉层中观察到相对较小的荧光强度。高粱碱提取的 β-D-葡聚糖（级分 2）显示，在 100 微克 ml-1 浓度下，10 分钟内大鼠腹膜巨噬细胞（体外）激活 30%。发现这种激活主要通过 PLA2 途径介导。在用级分2处理的小鼠体内碳清除试验中观察到吞噬指数k为0.102+/-0.008。与对照组相比，该组小鼠的脾和肝中胶体碳颗粒的积累是中等的。
Fluorescence of Calcofluor and Congo Red was observed in stained sections of sorghum grain (Sorghum bicolor (L.) Moench), especially in the sub-aleurone cells indicating the presence of mixed linkage beta-D-glucan. Relatively less fluorescence intensity was observed in the single layered (approximately 20 microns thick) aleurone. Alkali extracted beta-D-glucan (fraction 2) of sorghum showed 30% activation of rat peritoneal macrophages (in vitro) at 100 micrograms ml-1 concentration in 10 min. This activation was found mediated mainly through PLA2 pathway. A phagocytic index k of 0.102 +/- 0.008 was observed in vivo carbon clearance test in mice in the group treated with fraction 2. Accumulation of colloidal carbon particles in spleen and liver of mice was moderate in this group, compared to control.