STUDY ON THE UNPRIMED POLY (DA-DT) SYNTHESIS CATALYZED BY PREPARATIONS OF ESCHERICHIA-COLI DNA-POLYMERASE-I
STUDY ON THE UNPRIMED POLY (DA-DT) SYNTHESIS CATALYZED BY PREPARATIONS OF ESCHERICHIA-COLI DNA-POLYMERASE-I
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DOI：
10.1093/nar/6.7.2545
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发表时间：
1979-01-01
影响因子：
14.9
通讯作者：
SALGANIK, RI
中科院分区：
文献类型：
--
作者：
NAZARENKO, IA;BOBKO, LE;SALGANIK, RI
Evidence was obtained indicating that the initiation of poly (dA-dT)de novosynthesis is provided by deoxynucleoside diphosphete: oligonucleotide deoxynucleotidyl transferase (dNDP-transferas present in preparations of E.coli DNA polymerase I and capable of catalyzing the unprimed polymerization of dNDP. dNDP-transferase synthesyzes short oligonucleotides which form template-primer complexes repeatedly replicated by DNA polymerase I. This conclusion was based on the following observations : the abolition of the lag period of poly (dA-dT) synthesis by preincubation of DNA-polymerase I preparations with dADP and dTDP; the presence of oligo (dA-dT) among the preincubation products; the suppressive effect of dithiothreitol and N-ethylmaleimide (inhibitors of dNDP-transferase) on thede novo, but not on the primed synthesis of poly (dA-dT), catalyzed by preparations of DNA-polymerase I.
