Chemical and physical stimuli induce cardiomyocyte differentiation from stem cells
Chemical and physical stimuli induce cardiomyocyte differentiation from stem cells
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化学和物理刺激诱导心肌细胞从干细胞分化
DOI：
10.1016/j.bbrc.2009.01.173
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发表时间：
2009-04-10
影响因子：
3.1
通讯作者：
Chen, Huaiqing
中科院分区：
文献类型：
--
作者：
Ge, Dongxia;Liu, Xiaojing;Chen, Huaiqing
在这项研究中，我们研究了大鼠骨髓间充质干细胞（BM MSCs）的心肌细胞分化的干细胞与模拟心肌梗死的条件。梗塞大鼠心肌提取物含有梗塞后产生的生化因子。通过对干细胞施加1Hz的4%应变来模拟心脏收缩和舒张。我们发现，梗死心肌提取物或4%菌株各自单独可诱导BM-MSC的心肌细胞分化，如心肌细胞特异性基因包括α-肌动蛋白、连接蛋白43、Nkx2.5、MEF 2c、GATA 4、α-MHC和肌钙蛋白I的表达所示。此外，提取物和4%菌株的组合对心肌细胞分化的影响比单独处理的效果更强。我们的研究结果表明，这种体外模型系统模拟了梗死后的局部心脏环境线索，并可能有助于识别参与心肌细胞分化的生化和物理因素。(C)2009 Elsevier Inc. All rights reserved.
In this study we investigated cardiomyocyte differentiation of rat bone marrow-mesenchymal stem cells (BM MSCs) by treating the stem cells with conditions mimicking that of myocardial infarction. The extract from infarcted rat myocardium Contained the biochemical factors arising after infarction. The cardiac contraction and relaxation were simulated by applying 4% strain at 1 Hz to the stem cells. We found that the extract from infarcted myocardium or 4% strain each alone could induce cardiomyocyte differentiation of BM-MSCs, as shown by expression of cardiomyocyte-specific genes including alpha-actin, connexin 43, Nkx2.5, MEF2c, GATA4, alpha-MHC, and Troponin I. Furthermore, a combination of the extract and 4% strain had stronger effects on cardiomyocyte differentiation than what either treatment alone had. Our results Suggest that this in vitro model system Simulates the local cardiac environment Cues after infarction and may be useful in identifying the biochemical and physical factors involved in cardiomyocyte differentiation. (C) 2009 Elsevier Inc. All rights reserved.