Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation
Centrosome separation and central spindle assembly act in redundant pathways that regulate microtubule density and trigger cleavage furrow formation
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DOI：
10.1016/s1534-5807(03)00057-1
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发表时间：
2003-03-01
影响因子：
11.8
通讯作者：
Glotzer, M
中科院分区：
文献类型：
--
作者：
Dechant, R;Glotzer, M
有丝分裂纺锤体提供空间线索，协调胞质分裂与核分裂。然而，有丝分裂纺锤体介导这种空间调节的具体性质仍然不清楚，部分原因是有丝分裂纺锤体的不同方面似乎在不同的系统中具有沟诱导活性。我们在C.在线虫胚胎中，虽然纺锤体通常是沟的起始所必需的，但当后期中心体分离程度降低时，它就成为沟形成所必需的。微管密度的测量表明，沟槽的形成发生在微管密度的局部最小值附近。纺锤体伸长程度的降低或中心纺锤体的破坏导致卵裂沟的延迟形成。这些数据表明，减少微管密度触发卵裂沟的启动和证明，冗余机制直接有效形成的卵裂沟。
The mitotic spindle provides the spatial cue that coordinates cytokinesis with nuclear division. However, the specific property of the mitotic spindle that mediates this spatial regulation remains obscure, in part because different aspects of the mitotic spindle appear to have furrow inducing activity in different systems. We show that in C. elegans embryos, although the central spindle is usually dispensable for furrow initiation, it becomes essential for furrow formation when the extent of centrosome separation during anaphase is reduced. Measurements of microtubule density demonstrate that furrow formation occurs in the vicinity of a local minimum of microtubule density. Reduction of the extent of spindle elongation or disruption of the central spindle causes delayed formation of the cleavage furrow. These data suggest that reduced microtubule density triggers cleavage furrow initiation and demonstrate that redundant mechanisms direct efficient formation of the cleavage furrow.