Specificity of the Acute Tryptophan and Tyrosine Plus Phenylalanine Depletion and Loading Tests Part II: Normalisation of the Tryptophan and the Tyrosine Plus Phenylalanine to Competing Amino Acid Ratios in a New Control Formulation.
Specificity of the Acute Tryptophan and Tyrosine Plus Phenylalanine Depletion and Loading Tests Part II: Normalisation of the Tryptophan and the Tyrosine Plus Phenylalanine to Competing Amino Acid Ratios in a New Control Formulation.
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DOI：
10.4137/ijtr.s5169
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Richard DM
中科院分区：
文献类型：
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作者：
Badawy AA;Dougherty DM;Richard DM
目前的急性色氨酸 (Trp) 或酪氨酸 (Tyr) 加苯丙氨酸 (Phe) 消耗和负载的制剂会导致 Trp 或 Tyr + Phe 与竞争氨基酸 (CAA) 的比率出现不良下降，从而破坏了这些测试的特异性。支链氨基酸 (BCAA) 会导致这些意外的降低，在本研究中降低新的平衡对照配方中的含量可导致所有比率正常化。四组（每组 n = 12）成人每组接受四种 50 g 对照制剂中的一种，其中 BCAA 含量减少 0%（传统）、20%、30% 或 40%。在传统配方后的前 5 小时内，游离和总 [Trp]/[CAA] 和 [Phe + Tyr]/[BCAA + Trp] 比率均显着下降，但通过含有 40% BCAA 的配方后完全正常化。我们推荐后者作为平衡对照制剂，并建议调整消耗和加载制剂，以增强其对 5-HT 和儿茶酚胺的特异性。
Current formulations for acute tryptophan (Trp) or tyrosine (Tyr) plus phenylalanine (Phe) depletion and loading cause undesirable decreases in ratios of Trp or Tyr + Phe to competing amino acids (CAA), thus undermining the specificities of these tests. Branched-chain amino acids (BCAA) cause these unintended decreases, and lowering their content in a new balanced control formulation in the present study led to normalization of all ratios. Four groups (n = 12 each) of adults each received one of four 50 g control formulations, with 0% (traditional), 20%, 30%, or 40% less of the BCAA. The free and total [Trp]/[CAA] and [Phe + Tyr]/[BCAA + Trp] ratios all decreased significantly during the first 5 h following the traditional formulation, but were fully normalized by the formulation containing 40% less of the BCAA. We recommend the latter as a balanced control formulation and propose adjustments in the depletion and loading formulations to enhance their specificities for 5-HT and the catecholamines.